Magnesium oxide (MgO) was obtained from Sigma-Aldrich. Methanol and ethanol were purchased from VWR International, Spånga, Sweden. Celecoxib was purchased from 3Way Pharm Inc, Shanghai, China. Commercially available celecoxib (Celebra®, Pfizer, Brooklyn, NY, USA) was purchased from Apoteket Kronan, Apoteket AB, Solna, Sweden. All chemicals were used as received. Celebra® 100 mg capsules contained lactose monohydrate, sodium lauryl sulfate, povidone, croscarmellose sodium, and magnesium stearate.
Celebra
Manufactured by Pfizer
Sourced in United States, Puerto Rico, Brazil
Celebra is a laboratory equipment product manufactured by Pfizer. It is designed for use in research and scientific applications. The core function of Celebra is to provide a controlled environment for various experiments and analytical procedures.
Automatically generated - may contain errors
4 protocols using celebra
1
Formulation and Characterization of Celecoxib
2
Preparation and Evaluation of Goniothalamin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Goniothalamin was prepared according to a published procedure (de Fatima et al. 2005) (link) and it was emulsified with 1% Tween 80 (Synth, Diadema, SP, Brazil) and dissolved in PBS, pH 7.0 (Vendramini-Costa et al. 2014) . Celecoxib was obtained from CELEBRA (Pfizer Pharmaceuticals LLC, Caguas, Puerto Rico), and diluted in CMC 0.05% (Sozer et al. 2011) (link). The acute toxicity of GTN has been evaluated in a previous study (Vendramini-Costa et al. 2010) (link).
3
Hamster Model of Inflammatory Skin Disorder
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All procedures involving animals were approved by Universidade Federal do Ceará, Committee on ethical treatment of research animals (protocol 0033/2012). Surgical procedures and animal treatments were conducted in accordance with the Guidelines for Institutional Animal Care, and were approved by our local ethics committee for experimental use on the animals.
Eighty-four adult male golden hamsters weighing 100-150g were housed in temperature-controlled rooms and received water and food ad libitum. Drugs 5-FU (25 mg/mL; Fluoro-acil ® , Roche, Rio de Janeiro, Brazil); MTK (Singulair ® , Merck Sharp and Dohme, Hoddesdon, HRT, United Kingdom); MK 886 (MK-886 sodium salt hydrate-(M2692 SIGMA) >98% (HPLC) -Synonym: 3-[3-tert-Butylthio-1-(4chlorobenzyl)-5-isopropyl-1H-indol-2-yl]-2,2dimethylpropionic acid, sodium salt hydrate); celecoxib (Celebra ® , capsules com 200 mg, Pfizer, São Paulo, Brazil); tribromoethanol 2,5% (25 mg/mL).
Eighty-four adult male golden hamsters weighing 100-150g were housed in temperature-controlled rooms and received water and food ad libitum. Drugs 5-FU (25 mg/mL; Fluoro-acil ® , Roche, Rio de Janeiro, Brazil); MTK (Singulair ® , Merck Sharp and Dohme, Hoddesdon, HRT, United Kingdom); MK 886 (MK-886 sodium salt hydrate-(M2692 SIGMA) >98% (HPLC) -Synonym: 3-[3-tert-Butylthio-1-(4chlorobenzyl)-5-isopropyl-1H-indol-2-yl]-2,2dimethylpropionic acid, sodium salt hydrate); celecoxib (Celebra ® , capsules com 200 mg, Pfizer, São Paulo, Brazil); tribromoethanol 2,5% (25 mg/mL).
4
Extraction and Fractionation of Medicinal Plants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The plant material from M. elliptica was collected at the Experimental Station of the Mogi-Guaçu Biological Reserve (São Paulo state, Brazil) and the plant material from A. glandulosa was collected at Ipueiras (Tocantins, Brazil). The exsiccates were deposited at the Herbarium in Tocantins (HTO) of the Federal University of Tocantins in Porto National city, under number 3341.
The plant material was dried at room temperature, and subjected to grinding using a knife mill (Willye -NL-226-02). The obtained powder was extracted by maceration using hexane and methanol. The methanolic extract was concentrated on a rotary evaporator (54-Rotavapor R-220, brand: Büchi, with vacuum pump Vacuum Contoller V-805, brand: Büchi and water circulator), yielding a crude, highly viscous extract which was diluted in a methanol: water mixture (1:9 v/v) and subjected to the liquidliquid partition with ethyl acetate and n-butanol.
The drug celecoxib has been commercially available. In Brazil, only Pfizer (Celebra®) markets this drug. In addition, the drug doxorubicin has been commercially available. In Brazil, this drug is market by Libbs (Fauldoxo®) and by Pfizer (Adriblastine®). The solvents used in the preparation of extracts and fractions were all analytical grade obtained from Vetec.
The plant material was dried at room temperature, and subjected to grinding using a knife mill (Willye -NL-226-02). The obtained powder was extracted by maceration using hexane and methanol. The methanolic extract was concentrated on a rotary evaporator (54-Rotavapor R-220, brand: Büchi, with vacuum pump Vacuum Contoller V-805, brand: Büchi and water circulator), yielding a crude, highly viscous extract which was diluted in a methanol: water mixture (1:9 v/v) and subjected to the liquidliquid partition with ethyl acetate and n-butanol.
The drug celecoxib has been commercially available. In Brazil, only Pfizer (Celebra®) markets this drug. In addition, the drug doxorubicin has been commercially available. In Brazil, this drug is market by Libbs (Fauldoxo®) and by Pfizer (Adriblastine®). The solvents used in the preparation of extracts and fractions were all analytical grade obtained from Vetec.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!