Crl 2975

Human MM cell lines ARP1 and H929 were kind gifts from Prof. Zhiqiang Liu (Department of Physiology and Pathophysiology, School of Basic Medical Science, Tianjin Medical University). MM.1S and MM.1R cells were purchased from ATCC (CRL-2974 and CRL-2975, respectively). Mouse 5TMM3VT cells were donated by Dr. Wen Zhou (Xiangya School of Medicine, Central South University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education; Key Laboratory of Carcinogenesis, National Health and Family Planning Commission, Changsha, China). The cells were maintained at 37 °C with 5% CO₂ in RPMI 1640 media (#05-065-1A, Biological Industries, Beit Haemek, Israel) supplemented with 10% heat-inactivated fetal bovine serum (FBS; #04-002-1A, Biological Industries, Israel) and 1% penicillin/streptomycin.

Human MM1R cells (CRL-2975) were purchased from ATCC (Manassas, VA, USA) and grown in RPMI-1640 medium supplemented with 10% FBS (E.U Approved; South American Origin) and 1% Pen-Strep solution (Invitrogen, Carlsbad, CA, USA) at 37 °C in 5% CO₂. Cell viability was measured with the colorimetric 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Sigma Aldrich, St. Louis, MO, USA) as previously described [93 (link)]. In all experiments, untreated MM1R cells were used as control cells.

Human MM cell lines MM.1S, MM.1R cells were purchased from ATCC (CRL-2974 and CRL-2975, respectively), ARP1 and H929 cells were kind gifts from Dr. Siegfried Janz (University of Iowa, Iowa City, IA, USA) and mouse 5TMM3VT cells were donated by Dr. Wen Zhou (Xiangya School of Medicine, Central South University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education; Key Laboratory of Carcinogenesis, National Health and Family Planning Commission, Changsha, China). All the cells were cultured in RPMI-1640 medium (Biological Industries, Beit Haemek, Israel) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Biological Industries, Israel) and 1% penicillin/streptomycin at 37°C with 5% CO₂. Primary human CD138⁺ cells were collected from the blood samples of each participant and cultured in the same conditions as described above, which was approved by the ethics committees of Affiliated Hospital of Nanjing University of Chinese Medicine (No. 2018NL-KS13).