The MDA-MB-435 human melanoma cell line is a widely used laboratory tool for cancer research. It is a continuous cell line derived from a human metastatic melanoma. The cells can be cultured and used for various experimental purposes, such as studying tumor cell biology, evaluating anti-cancer therapies, and investigating the mechanisms of melanoma progression.
Example 12
U87MG human glioblastoma, MDA-MB-435 human melanoma and HT-29 human colorectal adenocarcinoma cell lines were purchased from ATCC, and was grown in Minimum Essential Medium, Leibovitz's L-15 Medium and McCoy's 5A Medium, respectively. All the cells were supplemented with 10% fetal bovine serum, 100 IU/mL penicillin, and 100 mg/mL streptomycin. Cells were grown in humidified atmosphere containing 5% CO2 at 37° C.
Example 14
U87MG human glioblastoma, MDA-MB-435 human melanoma and HT-29 human colorectal adenocarcinoma cell lines were purchased from ATCC, and was grown in Minimum Essential Medium, Leibovitz's L-15 Medium and McCoy's 5A Medium, respectively. All the cells were supplemented with 10% fetal bovine serum, 100 IU/mL penicillin, and 100 mg/mL streptomycin. Cells were grown in humidified atmosphere containing 5% CO2 at 37° C.
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