Exogenous cel mir 39 3p

Manufactured by Qiagen

Exogenous cel-miR-39-3p is a synthetic microRNA (miRNA) derived from the nematode Caenorhabditis elegans. It is commonly used as a spike-in control for normalization of miRNA expression analysis.

Automatically generated - may contain errors

Lab products found in correlation

Miscript preamp pcr kit, by Qiagen (2 mentions) Miscript 2 rt kit, by Qiagen (2 mentions) Exorneasy serum plasma midi kit, by Qiagen (2 mentions)

Spelling variants of this product

Cel-miR-39 (102 citations) Cel-miR-39-3p (42 citations)

2 protocols using exogenous cel mir 39 3p

Exosome RNA Isolation and miRNA Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA, including miRNAs, was isolated from serum exosomes using the commercial exoRNeasy serum plasma midi kit (Qiagen, Germany) according to the manufacturer’s instructions. Exogenous cel-miR-39-3p (Qiagen) was added to the samples to measure the RNA isolation efficiency, as determined by the manufacturer.
The complementary DNA (cDNA) was then synthesized from 100 ng of total RNA by using the miScript^® II RT kit (Qiagen), and miRNA preamplification was performed using the miScript^® PreAmp PCR kit (Qiagen) according to the manufacturer’s instructions.

Da-Silva C.C., Anauate A.C., Guirao T.P., Novaes A.D., Maquigussa E, & Boim M.A. (2022). Analysis of exosome-derived microRNAs as early biomarkers of lipopolysaccharide-induced acute kidney injury in rats. Frontiers in Physiology, 13, 944864.

+ Open protocol

+ Expand

Exosomal miRNA Isolation and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA, including miRNAs, was isolated from serum exosomes using the commercial exoRNeasy serum plasma midi kit (Qiagen, Germany) according to the manufacturer's instructions. Exogenous cel-miR-39-3p (Qiagen) was added to the samples to measure the RNA isolation e ciency, as determined by the manufacturer.
The complementary DNA (cDNA) was then synthesized from 100 ng of total RNA by using the miScript ® II RT kit (Qiagen), and miRNA preampli cation was performed using the miScript ® PreAmp PCR kit (Qiagen) according to the manufacturer's instructions.

Da-Silva C.C.S., Anauate A.C., Maquigussa E., Guirão T.P., Silva J.A.N.d., & Boim M.A. (2020). Analysis of Exosome-derived MicroRNAs as Early Biomarkers of Lipopolysaccharide-induced Acute Kidney Injury in Rats.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!