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Anti mouse igg and hrp linked antibody

Manufactured by Cell Signaling Technology
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Sourced in United States

The Anti-mouse IgG and HRP-linked Antibody is a secondary antibody that binds to mouse primary antibodies. It is conjugated to horseradish peroxidase (HRP), which can be used for detection in immunoassays and Western blotting.

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Lab products found in correlation

Pierce ip lysis buffer, by Thermo Fisher Scientific (2 mentions) Anti flag m2 monoclonal antibody, by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Pageruler prestained protein ladder, by Thermo Fisher Scientific (1 mentions) Ripa lysis and extraction buffer, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions) Mg132, by Merck Group (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) Anti vegfr2, by Cell Signaling Technology (1 mentions) Mlc2pt18 s19, by Cell Signaling Technology (1 mentions) Mlc2ps19, by Cell Signaling Technology (1 mentions) Anti mekk3, by R&D Systems (1 mentions) Anti α smooth muscle actin mouse monoclonal antibody clone 1a4, by Merck Group (1 mentions) Anti cd31 rat monoclonal antibody clone mec13.3, by BD (1 mentions) Anti ve cadherin, by Santa Cruz Biotechnology (1 mentions) P jnk, by Cell Signaling Technology (1 mentions) Supersignal west atto ultimate sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Ibright fl1500 imaging system, by Thermo Fisher Scientific (1 mentions) Sqstm1 p62, by Cell Signaling Technology (1 mentions) 3 methyladenine 3 ma, by Selleck Chemicals (1 mentions)

Close spelling variants of this product

Anti-mouse HRP-linked antibody Anti-mouse HRP-linked HRP-linked antibody HRP anti-mouse IgG (Mouse)

4 protocols using anti mouse igg and hrp linked antibody

1

Protein Detection and Quantification

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MG-132 was purchased from Sigma–Aldrich (SML1135, Missouri, USA). PageRuler™ Prestained Protein Ladder (10−180 kDa, 26616), RIPA Lysis and Extraction Buffer (89900) and Pierce IP Lysis Buffer (87787) were purchased from Thermo Fisher Scientific (Eugene, USA). Anti-mouse IgG, and HRP-linked antibody (1:3000, 7076) and anti-rabbit IgG, HRP-linked antibody (1:3000, 7074) were purchased from Cell Signaling Technology (MA, USA).
Zhu H., Gao Y., Liu L., Tao M., Lin X., Cheng Y., Shen Y., Xue H., Guan L., Zhao H., Liu L., Wang S., Yang F., Zhou Y., Liao H., Sun F, & Lin H. (2023). A novel TNKS/USP25 inhibitor blocks the Wnt pathway to overcome multi-drug resistance in TNKS-overexpressing colorectal cancer. Acta Pharmaceutica Sinica. B, 14(1), 207-222.
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2

Immunoblotting and Immunofluorescence Protocols

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Monoclonal anti-Flag antibody M2 (catalogue number F1804) (dilution 1:1,000) is from Sigma, anti-tubulin antibody was prepared from an anti-tubulin hybridoma clone (12G10) purchased from the Hybridoma Bank at the University of Iowa (1:10,000). Anti-HA antibody was purified from 12CA5 hybridoma27 (link) (1:20,000). p-JNK (#4668) (1:1,000), p-ERK1/2 (#9106) (1:2,000), anti-VEGFR2 (#2472) (1:1,000), p-p38 (#9211) (1:1,000), MLC2pT18/S19 (#3674) (1:1,000 for immunoblot, 1:200 for IF), MLC2pS19 (#3671) (1:1,000) anti-mouse IgG and HRP-linked Antibody (#7076) (1:5,000) were purchased from Cell Signaling Technology (MA). Anti-MEKK3 is from R&D (MN) (MAB6095) (1:1,000), and anti-VE-Cadherin is from Santa Cruz Biotechnology (Dallas, TX) (sc-79818) (1:200), anti-α-smooth muscle actin mouse monoclonal antibody (clone 1A4) is from Sigma (A5228) (1:200) and anti-CD31 rat monoclonal antibody (clone MEC13.3) is from BD bioscience (553370) (1:200).
Fisher O.S., Deng H., Liu D., Zhang Y., Wei R., Deng Y., Zhang F., Louvi A., Turk B.E., Boggon T.J, & Su B. (2015). Structure and vascular function of MEKK3–cerebral cavernous malformations 2 complex. Nature Communications, 6, 7937.
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3

Immunoblot Analysis of Protein Targets

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Immunoblot analysis was performed as described previously (Jin et al. 2021 (link)). Primary antibodies for β-tubulin, FLAG-tag, and GFP used in this study were monoclonal anti-β-tubulin antibody (clone: 10G10; Fujifilm), monoclonal anti-FLAG M2 antibody (F1804; Sigma-Aldrich), and monoclonal anti-GFP antibodies (clones: 7.1 and 13.1; 11814460001; Roche), respectively. Secondary antibodies were anti-mouse IgG and HRP-linked Antibody (7076S; Cell Signaling Technology, MA, USA). Chemiluminescence signals produced by ImmunoStar Zeta (Fujifilm) or SuperSignal West Atto Ultimate Sensitivity Substrate (Thermo Fisher Scientific) were detected using a CCD camera system (iBright FL1500 Imaging System; Thermo Fisher Scientific). Band intensities were quantified using Fiji Gel Analyzer.
Jin J., Iwama R, & Horiuchi H. (2023). The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans. Current Genetics, 69(2-3), 175-188.
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4

Xanthatin Modulates Cell Death Pathways

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Xanthatin dissolved with dimethyl sulfoxide (DMSO) to 50 mM and stored at -20°C. MTS was purchased from Promega (USA). 5-Fluorouracil (5-FU) was purchased from MCE (USA).
3-Methyladenine (3-MA) was purchased from Selleck (USA). NAC was purchased from Beyotime (Shanghai, China) . Antibodies against cleaved caspase-3 (Asp175), caspase-3, cleaved caspase-7 (Asp198), caspase-7, cleaved PARP (Asp214), PARP, LC3B, SQSTM1/p62, XIAP, GAPDH, and anti-mouse IgG and HRP-linked antibody were all purchased from Cell Signaling (Beverly, MA, USA). DMEM and DME/F12 were obtained from Thermo Fisher Scientific (NY, USA) and fetal bovine serum was obtained from Sijiqing (Hangzhou, china).
Geng Y.D., Zhang L., Wang G.Y., Feng X.J., Chen Z.L., Jiang L, & Shen A.Z. (2020). Xanthatin mediates G2/M cell cycle arrest, autophagy and apoptosis via ROS/XIAP signaling in human colon cancer cells. Natural product research, 34(18).
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