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Anti mouse cd3ε buv737

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Anti-mouse CD3ε-BUV737 is a fluorescently labeled antibody that binds to the CD3ε subunit of the T cell receptor complex in mouse samples. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Anti mouse tnfα bv605, by BioLegend (2 mentions) Anti mouse tcrβ af700, by BioLegend (2 mentions) Anti mouse tcr γ δ fitc antibody, by BioLegend (2 mentions) Anti mouse f4 80 af488, by BioLegend (2 mentions) Anti mouse b220 alexa fluor 700, by BioLegend (2 mentions) Anit mouse ly6c, by BioLegend (2 mentions) Anti mouse cd11b buv737, by BD (2 mentions) Intracellular staining buffer set, by Thermo Fisher Scientific (2 mentions) Anti mouse il 17a pacific blue, by BioLegend (2 mentions) Anti mouse il 36α apc, by Assaypro (2 mentions) Anti mouse cd8α pe, by BioLegend (2 mentions) Anti mouse nk1.1 apc, by BioLegend (2 mentions) Anti mouse 1 a 1 e bv605, by BioLegend (2 mentions) Anti mouse cd16 32, by BioLegend (2 mentions) Pe anti mouse cd11c, by BioLegend (2 mentions) Facsaria 2 cell sorter, by BD (2 mentions) Facs fortessa, by BD (2 mentions)

2 protocols using anti mouse cd3ε buv737

1

Multiparametric Flow Cytometry Analysis

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For surface staining, immune cells in skin tissues were first blocked with anti-mouse CD16/32 (101302, Biolegend) for 30 min at 4°C in PBS containing 1% FBS. After washing, cells were stained with following antibodies: anti-mouse TCRβ-AF700(109224, Biolegend), anti-mouse TCR γ/δ-FITC antibody (118106, Biolegend), anti-mouse CD8α-PE (100708, Biolegend), anti-mouse CD11c-PE (117308, Biolegend), anti-mouse F4/80-AF488 (123120, Biolegend), anti-mouse NK1.1-APC(108710, Biolegend), anti-mouse B220-Alexa Fluor 700 (103232, Biolegend), anit-mouse Ly6C (128026, Biolegend), anti-mouse I-A/I-E-BV605 (107639, Biolegend), anti-mouse CD3ε-BUV737 (612771, BD Bioscience), anti-mouse CD11b-BUV737 (612800, BD Bioscience; For intracellular staining, cells were washed with PBS, fixed and permeabilized with Intracellular Staining Buffer Set (00-5523-00, Invitrogen) according to the manufacturer’s protocol. Cells were stained for 1 h at 4°C with different antibodies: anti-mouse TNFα-BV605 (506329, Biolegend), anti-mouse IL-17A-Pacific blue (506918, Biolegend), anti-mouse IL-36α-APC (32103-05161, Assaypro), anti-mouse IL-36γ (PAL621Mu01, Cloud-clone Corp), or control antibody (goat anti-rabbit IgG, 111-136-144, Jackson ImmunoResearch). Cells were acquired either by BD FACS Fortessa or BD FACS Aria II Cell Sorter. All the data were analyzed using FlowJo 10.1.
Tamaru Y, & Doi R.H. (1999). Three Surface Layer Homology Domains at the N Terminus of the Clostridium cellulovorans Major Cellulosomal Subunit EngE. Journal of Bacteriology, 181(10), 3270-3276.
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2

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For surface staining, immune cells in skin tissues were first blocked with anti-mouse CD16/32 (101302, Biolegend) for 30 min at 4°C in PBS containing 1% FBS. After washing, cells were stained with following antibodies: anti-mouse TCRβ-AF700(109224, Biolegend), anti-mouse TCR γ/δ-FITC antibody (118106, Biolegend), anti-mouse CD8α-PE (100708, Biolegend), anti-mouse CD11c-PE (117308, Biolegend), anti-mouse F4/80-AF488 (123120, Biolegend), anti-mouse NK1.1-APC(108710, Biolegend), anti-mouse B220-Alexa Fluor 700 (103232, Biolegend), anit-mouse Ly6C (128026, Biolegend), anti-mouse I-A/I-E-BV605 (107639, Biolegend), anti-mouse CD3ε-BUV737 (612771, BD Bioscience), anti-mouse CD11b-BUV737 (612800, BD Bioscience; For intracellular staining, cells were washed with PBS, fixed and permeabilized with Intracellular Staining Buffer Set (00-5523-00, Invitrogen) according to the manufacturer’s protocol. Cells were stained for 1 h at 4°C with different antibodies: anti-mouse TNFα-BV605 (506329, Biolegend), anti-mouse IL-17A-Pacific blue (506918, Biolegend), anti-mouse IL-36α-APC (32103-05161, Assaypro), anti-mouse IL-36γ (PAL621Mu01, Cloud-clone Corp), or control antibody (goat anti-rabbit IgG, 111-136-144, Jackson ImmunoResearch). Cells were acquired either by BD FACS Fortessa or BD FACS Aria II Cell Sorter. All the data were analyzed using FlowJo 10.1.
Hao J., Jin R., Zeng J., Hua Y., Yorek M.S., Liu L., Mandal A., Li J., Zheng H., Sun Y., Yi Y., Yin D., Zheng Q., Li X., Ng C.K., Rouchka E.C., Egilmez N.K., Jabbari A, & Li B. (2022). Consumption of fish oil high-fat diet induces murine hair loss via epidermal fatty acid binding protein in skin macrophages. Cell reports, 41(11), 111804.
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