ICD was characterized by surface calreticulin expression and ATP and HMGB1 secretion in cell supernatant. Calreticulin staining. CT26 cells were incubated with peptide (2 µΜ) or micelles (10 µΜ) for 24 hours, lifted with Accutase, and stained with anti-calreticulin primary antibody (1:20, Abcam) followed by an anti-mouse secondary antibody (1:750), and analyzed on an Attune NxT (Invitrogen) flow cytometer. ATP release. CT26 cells were incubated with peptide or micelles at indicated concentrations for 24 hours and supernatant was collected and assessed for ATP concentration via the ENLITEN ATP Assay (Promega) according to manufacturer instructions. HMGB1 release. CT26 cells were incubated with peptide (2 µΜ) or micelles (12.5 µΜ) for 48 hours and supernatant was collected. Insoluble lipids were removed via centrifugation. HMGB1 concentration was assessed via ELISA (Chondrex) according to manufacturer instructions.
Anti calreticulin primary antibody
Manufactured by Abcam
Sourced in United States
Anti-calreticulin primary antibody is a laboratory reagent used to detect and study the presence and distribution of the calreticulin protein in biological samples. Calreticulin is a calcium-binding protein involved in multiple cellular processes. This antibody can be utilized in various techniques, such as Western blotting and immunohistochemistry, to identify and analyze the calreticulin protein.
Automatically generated - may contain errors
Lab products found in correlation
Attune nxt, by Thermo Fisher Scientific (1 mentions)
Enliten atp assay, by Promega (1 mentions)
Pluronic f 127, by Merck Group (1 mentions)
β actin mouse monoclonal antibody, by Beyotime (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
Anti cd3 fitc, by Proteintech (1 mentions)
Hrp conjugated goat anti rabbit igg h l, by Beyotime (1 mentions)
Hrp conjugated goat anti mouse igg h l, by Beyotime (1 mentions)
Alexa fluor 488 labeled goat anti rabbit igg h l, by Beyotime (1 mentions)
2 protocols using anti calreticulin primary antibody
1
Characterizing Immunogenic Cell Death Markers
2
Atorvastatin and Cyclophosphamide Combination Therapy
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Atorvastatin (ATO, catalog number: BD18106) and cyclophosphamide (CTX, catalog number: BD122996) were purchased from Bidepharm (China). Pluronic F127 was purchased from Sigma-Aldrich (United States). ATP Assay Kit (catalog number: S0026) was purchased from Beyotime biotechnology (China). Anti-CD16/32 (catalog number: 65080-1-L), anti-CD3-FITC (catalog number: FITC-65077), and anti-CD8a-APC (catalog number: APC-65069) were purchased from Proteintech (China). Anti-CD274 (PD-L1)-PE (catalog number: 124308) was purchased from Biolegend (United States). Anti-calreticulin primary antibody (catalog number: ab271865) was purchased from Abcam (United States). HMGB1 rabbit monoclonal antibody (catalog number: AG2167), β-actin mouse monoclonal antibody (catalog number: AF0003), Alexa Fluor 488-labeled goat anti-rabbit IgG (H + L) (catalog number: A0423), HRP-conjugated goat anti-mouse IgG (H + L) (catalog number: A0216), and HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number: A0208) were purchased from Beyotime biotechnology (China).
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