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Sapanisertib

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Sapanisertib is a small-molecule inhibitor that targets the serine/threonine protein kinase mTOR (mammalian target of rapamycin). mTOR is a key regulator of cell growth, proliferation, and survival.

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Lab products found in correlation

Torin1, by Bio-Techne (2 mentions) Azd5363, by Selleck Chemicals (2 mentions) Pan akt, by Cell Signaling Technology (2 mentions) P70 s6 kinase, by Cell Signaling Technology (2 mentions) Fr180204, by Bio-Techne (2 mentions) Phospho p44 42 mapk, by Cell Signaling Technology (2 mentions) Phospho s6 ribosomal protein, by Cell Signaling Technology (2 mentions) Phospho p70 s6 kinase thr389, by Cell Signaling Technology (2 mentions) S6 ribosomal protein, by Cell Signaling Technology (2 mentions) Pd980595, by Bio-Techne (2 mentions) P44 42 mapk, by Cell Signaling Technology (2 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (2 mentions) U0126, by Bio-Techne (2 mentions) 4e bp1, by Cell Signaling Technology (2 mentions) Pakt s473, by Cell Signaling Technology (2 mentions) Bkm120, by Novartis (2 mentions) Rapalink 1, by MedChemExpress (2 mentions) Capivasertib, by Selleck Chemicals (1 mentions) Rad001, by MedChemExpress (1 mentions) Human foreskin fibroblast cells, by American Type Culture Collection (1 mentions)

3 protocols using sapanisertib

1

Western Blotting Antibody Panel

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The following antibodies for the purpose of Western blotting were acquired from Cell Signaling Technologies: pAKT S473 (Catalog number 4060), PAN-AKT (#2920), Phospho-p44/42 MAPK (#4370), p44/42 MAPK (#4695), S6 Ribosomal Protein (#2317), Phospho-S6 Ribosomal Protein (#4858), Phospho-p70S6 Kinase Thr389 (#97596), p70S6 Kinase (#9202), Phospho-4EBP1 Thr37/46 (#2855), 4EBP1 (#9452). Antibodies against Beta-Actin were purchased from GeneTex (catalog number 109639). MAPK inhibitors were purchased from Tocris: FR180204 (catalog number 3706), U0126 (catalog number 1144), PD980595 (catalog number 1213). Sapanisertib (Cat# HY-13328) and Rapalink-1 (Cat# HY-111373) were purchased from MedChemExpress. Torin-1 (Cat #4247) was purchased from Tocris. PI3K/AKT/mTOR inhibitors BKM120 (Buparlisib; Novartis), AZD5363 (Capivasertib; Selleckchem), and RAD001 (Everolimus, MedChemExpress Cat# 159351–69–6) were generously provided by Dr Kathleen Millen.
Wehle D.T., Bass C.S., Sulc J., Mirzaa G, & Smith S.E. (2023). Protein interaction network analysis of mTOR signaling reveals modular organization. The Journal of Biological Chemistry, 299(11), 105271.
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2

Antibody Validation for Western Blotting

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The following antibodies for the purpose of western blotting were acquired from Cell Signaling Technologies: pAKT S473 (Catalog number 4060), PAN-AKT (#2920), Phospho-p44/42 MAPK (#4370), p44/42 MAPK (#4695), S6 Ribosomal Protein (#2317), Phospho-S6 Ribosomal Protein (#4858), Phospho-p70S6 Kinase Thr389 (#97596), p70S6 Kinase (#9202), Phospho-4EBP1 Thr37/46 (#2855), 4EBP1 (#9452). Antibodies against Beta-Actin were purchased from GeneTex (catalog number 109639). MAPK inhibitors were purchased from Tocris: FR180204 (catalog number 3706), U0126 (catalog number 1144), PD980595 (catalog number 1213). Sapanisertib (Cat# HY-13328) and Rapalink-1 (Cat# HY-111373) were purchased from MedChem Express. Torin-1 (Cat #4247) was purchased from Tocris. PI3K/AKT/mTOR inhibitors BKM120 (Buparlisib; Novartis, Switzerland), AZD5363 (Capivasertib; Selleckchem, USA), and RAD001 (Everolimus, Chem Express Cat# 159351–69-6) were generously provided by Kathleen Millen.
Wehle D.T., Bass C.S., Sulc J., Mirzaa G, & Smith S.E. (2023). Protein interaction network analysis of mTOR signaling reveals modular organization. bioRxiv.
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3

ATL and ALCL Cell Lines Protocol

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Upadacitinib (ABT494), AZD8055 and Sapanisertib were purchased from Medchem Express (Monmouth Junction, NJ). Human IL-2-dependent ATL cell lines; ED40515(+), ED41214(+), ATL55T(+) were kindly provided by Dr. Michiyuki Maeda (Kyoto University), KOB, LM-Y1, KK1 were provided by Dr. Yasuaki Yamada (Nagasaki University). Cells were maintained in RPMI 1640 medium plus 10% FBS with 100U/mL recombinant human IL-2. Human IL-2- independent ATL cell lines; ED40515(-), ED41214(-) and ATL43Tβ(-) were obtained from (Dr. Michiyuki Maeda, Kyoto University), ST1 (Dr. Tomoko Hata, Nagasaki University), Su9T01 (Dr. Naomichi Arima, Kagoshima University), ATN1 (Dr. Tomoki Naoe, Nagoya University) and MT1 (Dr. Miyoshi, Okayama University) and ALK(−) Anaplastic large cell lymphoma (ALCL) cell lines; FEPD, Mac-1, Mac2A, Mac2B, TLBR1, TLBR2 were maintained in RPMI complete medium without cytokine. Human foreskin fibroblast cells were purchased from ATCC (Gaithersburg, MD).
Daenthanasanmak A., Lin Y., Zhang M., Bryant B.R., Petrus M.N., Bamford R.N., Thomas C.J., Miljkovic M.D., Conlon K.C, & Waldmann T.A. (2020). Enhanced efficacy of JAK1 inhibitor with mTORC1/C2 targeting in smoldering/chronic adult T cell leukemia. Translational Oncology, 14(1), 100913.
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