Lsm 510 confocal laser scanning microscope system

Manufactured by Zeiss

Sourced in Germany

The LSM-510 is a confocal laser scanning microscope system manufactured by Zeiss. The core function of the LSM-510 is to provide high-resolution optical sectioning and imaging capabilities for various applications in microscopy.

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Lab products found in correlation

Fluorescent microscope, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

Close spelling variants of this product

LSM 510 META NLO Two-Photon Laser Scanning Confocal Microscope System LSM 510 confocal laser scanning microscope LSM 510 confocal laser scanning system LSM 510 laser scanning microscope LSM 510 laser confocal microscope LSM 510 confocal scanning microscope Laser Scanning Systems LSM 510 LSM 510 laser scanning system 510 confocal laser scanning microscope LSM confocal laser-scanning microscope

4 protocols using lsm 510 confocal laser scanning microscope system

Immunofluorescence Staining Protocol

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Cells seeded on glass slides were washed with PBS and fixed in 4% PFA for 15 min. Fixed cells were permeabilized with PBS containing 0.1% Triton X-100 for 15 min and blocked with 1 mg/ml bovine serum albumin in PBS for 30 min at room temperature. Primary antibodies were incubated for 3 h at room temperature or overnight at 4 °C and secondary antibodies were incubated at room temperature for 1 h. DAPI was dyed for visualizing nuclei. The images were taken under an LSM-510 confocal laser scanning microscope system (Carl Zeiss).

Ye X., Wu H., Sheng L., Liu Y.X., Ye F., Wang M., Zhou H., Su Y, & Zhang X.K. (2019). Oncogenic potential of truncated RXRα during colitis-associated colorectal tumorigenesis by promoting IL-6-STAT3 signaling. Nature Communications, 10, 1463.

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Immunofluorescence Staining of Cells and Tissues

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Cells mounted on glass slides or tumour tissue frozen sections (5-μm-thick) were washed with PBS and fixed in 4% paraformaldehyde (PFA) for 15 min and permeabilized with PBS containing 0.1% Triton X-100 for 15 min. Fixed cells or tumour tissues were blocked with 5 mg ml⁻¹ BSA in PBS for 30 min at room temperature, followed with incubation with primary antibodies for 3 h and secondary antibodies at room temperature for 1 h and co-stained with 4′6′-diamidino-2-phenylindole (DAPI) to visualize nuclei. The images were taken under an LSM-510 confocal laser scanning microscope system (Carl Zeiss).

Chen L., Aleshin A.E., Alitongbieke G., Zhou Y., Zhang X., Ye X., Hu M., Ren G., Chen Z., Ma Y., Zhang D., Liu S., Gao W., Cai L., Wu L., Zeng Z., Jiang F., Liu J., Zhou H., Cadwell G., Liddington R.C., Su Y, & Zhang X.K. (2017). Modulation of nongenomic activation of PI3K signalling by tetramerization of N-terminally-cleaved RXRα. Nature Communications, 8, 16066.

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Confocal Microscopy Analysis of Protein Localization

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Confocal microscopy was conducted as described (Kolluri et al., 2008 (link); Li et al., 2000 (link); Lin et al., 2004 (link)). Cells mounted on glass slides were permeabilized with PBS containing 0.1% Triton X-100 and 0.1 mol/L glycine for 15 min, and blocked with 1% bovine serum in PBS for 30 min at room temperature, followed with incubation with various primary antibodies at room temperature for 3 hr, and detected by FITC-labeled anti-IgG (1:400), anti-goat IgG conjugated with Cy3(1:400), or Cy5-labeled antibody at room temperature for 1 hr. Cells were costained with 4′,6-diamidino-2-phenylindole (DAPI) (1:10000 dilution) to visualize nuclei. The images were taken under a fluorescent microscope (CarlZeiss) or an LSM-510 confocal laser scanning microscope system (CarlZeiss). HepG2 cells, MEFs, and Nur77^−/− MEFs were treated with celastrol (1 μM or 4 μM) for the indicated time before exposed to TNFα (20 ng/mL) for additional 30 min. Mitochondria were marked by Mitotracker (Red) (1:10000 dilution) for 30 min before fixed by 4% buffered formalin/PBS. Hsp60, Ub, p65, TRAF2, and Nur77 were examined by immunostaining.

Hu M., Luo Q., Alitongbieke G., Chong S., Xu C., Xie L., Chen X., Zhang D., Zhou Y., Wang Z., Ye X., Cai L., Zhang F., Chen H., Jiang F., Fang H., Yang S., Liu J., Diaz-Meco M.T., Su Y., Zhou H., Moscat J., Lin X, & Zhang X.K. (2017). Celastrol-Induced Nur77 Interaction with TRAF2 Alleviates Inflammation by Promoting Mitochondrial Ubiquitination and Autophagy. Molecular cell, 66(1), 141-153.e6.

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Immunofluorescence Staining of Cells

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Cells mounted on glass slides were permeabilized with PBS containing 0.1% Triton X-100 for 15 min, and blocked with 1% bovine serum albumin (BSA) in PBS for 30 min at room temperature, followed with incubation with various primary antibodies at 37°C for 1 hr and detected by FITC-labeled anti-mouse/rabbit IgG (Chemicon International) or anti-mouse/rabbit IgG conjugated with Cy3 (Chemicon International) at room temperature for 30 min. Cells were co-stained with 4’6’-diamidino-2-phenylindole (DAPI) (Sigma) to visualize nuclei. The images were taken under an LSM-510 confocal laser scanning microscope system (Carl Zeiss, Oberkochen, Germany).

Liu J., Wang G.H., Duan Y.H., Dai Y., Bao Y., Hu M., Zhou Y.Q., Li M., Jiang F., Zhou H., Yao X.S, & Zhang X.K. (2017). Modulation of the Nur77-Bcl-2 apoptotic pathway by p38α MAPK. Oncotarget, 8(41), 69731-69745.

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