Quantikine elisa human hepcidin immunoassay

Manufactured by R&D Systems

Sourced in United States

The Quantikine ELISA Human Hepcidin Immunoassay is a quantitative sandwich enzyme immunoassay designed to measure human hepcidin levels in serum, plasma, and cell culture supernatants. It is a laboratory tool used for the quantitative determination of human hepcidin concentrations.

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Jca bm8060, by JEOL (1 mentions)

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Quantikine ELISA (398 citations) ELISAs (144 citations) Quantikine Human Immunoassay (17 citations) Quantikine Immunoassays (15 citations) Hepcidin (7 citations) Quantikines (4 citations) Quantikine Human (2 citations)

2 protocols using quantikine elisa human hepcidin immunoassay

Biomarker Analysis of Japanese Adults

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Blood was collected from the cubital vein in the morning before eating breakfast.
Serum albumin, creatine kinase (CK), ferritin, iron, and total iron-binding capacity (TIBC) were analyzed using a JCA-BM8060 automated analyzer (Japan Electron Optics Laboratory Ltd., Tokyo, Japan). Hematologic parameters, including white blood cell, red blood cell, hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume, mean corpuscular hemoglobin (MCH), MCH concentration, and platelet (PLT), were analyzed using a Sysmex XE 2100 automated hematology analyzer (Sysmex Corporation, Kobe, Japan). A commercial laboratory service, Hoken Kagaku Inc. (Yokohama, Japan), conducted the analyses and provided reference ranges of healthy Japanese male adults.
Serum hepcidin was analyzed using a commercially available enzyme immunoassay kit (Quantikine ELISA Human Hepcidin Immunoassay, R&D Systems, Minneapolis, MN, USA). The sample range was provided by the manufacturer.
TSAT was calculated as serum iron/TIBC. The reference range was obtained from the MDS Manual Professional Edition.

Tokuyama M., Seino J., Sakuraba K, & Suzuki Y. (2021). Possible Association of Energy Availability with Transferrin Saturation and Serum Iron during Summer Camp in Male Collegiate Rugby Players. Nutrients, 13(9), 2963.

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Serum Biomarker Quantification Protocol

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Sample serum concentrations of CD163, ferritin, and hepcidin were measured using 4 assays: Human CD163 (M130) ELISA kit (ThermoScientific Pierce, EHCD163), Human Ferritin ELISA Kit (ThermoScientific Pierce, EHFTL), and Quantikine ELISA Human Hepcidin Immunoassay (R&D Systems, DHP250) according to the manufacturer’s instructions. Serum samples were diluted per manufacturer instructions. Absorbance measurements were recorded at 450–550 nm using a spectrophotometer. Standards curves were created for CD163, hepcidin, and ferritin using prepared standard concentrations with R software (version 3.3.0, 2016). For ferritin and CD163, one and two outliers respectively were eliminated from the standard concentration curve in order to establish a best fit. Sample concentrations were then calculated based on the standard curves.

Garton A.L., Gupta V.P., Christophe B.R, & Connolly ES J.r. (2017). Biomarkers of functional outcome in intracerebral hemorrhage: Interplay between clinical metrics, CD163, and ferritin. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 26(8), 1712-1720.

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