An anti-β-actin mouse monoclonal antibody (cat. no. A5441; 1:6,000), 4HR (cat. no. 209465), tartrate resistant acid phosphatase (TRAP) staining kit (cat. no. 387A) were purchased from Sigma-Aldrich; Merck KGaA. Rabbit polyclonal anti-TRAP (cat. no. 11594-1-AP; 1:1,000), Mouse monoclonal anti-nuclear factor of activated T-cells cytoplasmic 1 (NFATc1; cat. no. 66963-1-Ig; 1:1,000) and rabbit polyclonal anti-Cathepsin K (cat. no. 11239-1-AP; 1:1,000) antibodies were from ProteinTech Group, Inc. Rabbit monoclonal anti-phosphorylated (p)-IκB kinase β (pIKKβ; cat. no. 2697S, 1:1,000) and total anti-IKKβ rabbit monoclonal (cat. no. 8943S; cat. no. 1:1,000) antibodies were obtained from Cell Signaling Technology, Inc. Recombinant soluble M-CSF and RANKL were obtained from PeproTech, Inc. Cell Counting Kit-8 (CCK-8) kit was purchased from Wuhan Boster Biological Technology, Ltd. Serum cross-linked procollagen type 1amino-terminal propeptide (P1NP, cat. no. SEA570Mu) and C-terminal telopeptide of type1 collagen (CTX1, cat. no. CEA665Mu) ELISA kits were purchased from Cloud-Clone Corp.
Cell counting kit 8 cck 8 kit
Manufactured by Wuhan Boster Biological Technology
The Cell Counting Kit-8 (CCK-8) is a colorimetric assay kit used for the determination of cell viability and cytotoxicity. The kit utilizes the highly water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to produce a yellow-colored formazan dye. The amount of the formazan dye generated is directly proportional to the number of living cells.
Automatically generated - may contain errors
Lab products found in correlation
Rankl, by Thermo Fisher Scientific (1 mentions)
Streptozocin stz, by Merck Group (1 mentions)
α smooth muscle actin α sma, by Proteintech (1 mentions)
Proliferating cell nuclear antigen (pcna), by Proteintech (1 mentions)
Anti cse, by Proteintech (1 mentions)
Enhanced chemiluminescence ecl reagent, by Beyotime (1 mentions)
Pd98059, by Merck Group (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
Sv40 mes13, by Shanghai Cell Bank (1 mentions)
2 protocols using cell counting kit 8 cck 8 kit
1
Osteoclastogenesis Regulation via IKKβ Signaling
2
SV40-Immortalized Mouse Renal Mesangial Cells
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Mouse renal MC lines (SV40-MES13) were purchased from Shanghai Cell Bank of Chinese Academy of Science. SKF38393 (DR1 agonist), sodium hydrogen sulfide (NaHS), PPG (a CSE inhibitor), PD98059 (an ERK1/2 inhibitor), streptozocin (STZ) and 7-Azido-4-Methylcoumarin (H2S probe) were purchased from Sigma-Aldrich (Merck KGaA). The primary antibodies for anti-CSE, cyclin D1, PCNA, P21, COL1, α-smooth muscle actin (α-SMA), and MMP9 were from ProteinTech Group, Inc. DR1 antibody was purchased from GeneTex, Inc. The total (t)-ERK1/2 and phosphorylated (p)-ERK1/2 antibodies were obtained from Cell Signaling Technology, Inc. The Cell Counting Kit-8 (CCK-8) kit and the primary antibody for β-actin were obtained from Wuhan Boster Biological Technology, Ltd. The EdU kit was obtained from Guangzhou RiboBio Co., Ltd. The BCA Protein Assay kit and Enhanced Chemiluminescence (ECL) reagent were purchased from Beyotime Institute of Biotechnology.
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