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Bhi broth

Manufactured by Eiken Chemical
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Sourced in Japan

BHI broth is a general-purpose microbiological growth medium that supports the cultivation of a wide range of bacteria. It is composed of brain heart infusion, which provides nutrients for the growth of a variety of microorganisms.

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Lab products found in correlation

Bacitracin, by Fujifilm (1 mentions) Gramicidin d, by Fujifilm (1 mentions) Yeast extract, by Fujifilm (1 mentions)

2 protocols using bhi broth

1

Preparation of P. aeruginosa Inoculum

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P. aeruginosa (PAO-1 strain) were prepared as described previously [45 (link)]. Briefly, P. aeruginosa were inoculated onto a sheep blood agar plate and incubated at 37 °C overnight, and colonies were cultured in brain heart infusion (BHI) broth (Eiken Chemical Co., Ltd., Tokyo, Japan) at 37 °C for 24 h and washed three times in normal saline. After the final suspension was mixed, bacterial counts were performed by measuring the absorbance at 600 nm. In each experiment, a quantification culture was performed to confirm the inoculation dose.
Tanno H., Kanno E., Sato S., Asao Y., Shimono M., Kurosaka S., Oikawa Y., Ishi S., Shoji M., Sato K., Kasamatsu J., Miyasaka T., Yamamoto H., Ishii K., Imai Y., Tachi M, & Kawakami K. (2021). Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds. International Journal of Molecular Sciences, 22(8), 3931.
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2

Bacterial Cultivation Conditions

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Facultative anaerobic bacteria, S. mutans ATCC 35668 and A. actinomycetemcomitans ATCC 29522, and obligate anaerobic bacteria, P. gingivalis ATCC 33277, were kept frozen until analysis. The stocks were incubated in brain heart infusion (BHI) broth (Pearlcore®, Eiken Chemical Co. Ltd., Tokyo, Japan) supplemented with 0.1% antibiotic (gramicidin D and bacitracin, Wako Pure Chemical Industries Ltd.) and 1% sucrose for S. mutans; 1% yeast extract (Wako Pure Chemical Industries Ltd.) for A. actinomycetemcomitans; and 0.5% yeast extract, 0.0005% hemin, and 0.0001% menadione for P. gingivalis.
Miyata S., Miyaji H., Kawasaki H., Yamamoto M., Nishida E., Takita H., Akasaka T., Ushijima N., Iwanaga T, & Sugaya T. (2017). Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation. International Journal of Nanomedicine, 12, 2703-2716.
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