Mirna mimics or inhibitors

Manufactured by RiboBio

Sourced in China

MiRNA mimics or inhibitors are synthetic molecules designed to modulate the expression of specific microRNAs (miRNAs) in biological systems. These products are used for research purposes to study the functional roles of miRNAs in cellular processes. The core function of miRNA mimics is to increase the expression of a target miRNA, while miRNA inhibitors are used to decrease the expression of a target miRNA. These products are intended for use in in vitro and in vivo experimental settings to investigate the effects of miRNA modulation on gene expression, cellular physiology, and disease-related pathways.

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Lab products found in correlation

Fetal bovine serum (fbs), by Lonza (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Ribofect transfection kit, by RiboBio (1 mentions) Hepg2, by RiboBio (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Hyclone, by Cytiva (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Nc sirna, by Thermo Fisher Scientific (1 mentions) Turbofect transfection reagent, by Thermo Fisher Scientific (1 mentions) Pmirglo, by Promega (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MiRNA mimics (108 citations) MiRNA inhibitors (47 citations) MiRNA mimic/inhibitor (5 citations) MiRNA mimic or inhibitor (2 citations)

4 protocols using mirna mimics or inhibitors

Transfection and Luciferase Assay of miR-589-5p

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SRA01/04 cell line originated from human lens epithelium was bought from Chinese Academy of Sciences (Shanghai, China). The cells were cultured in Dulbecco’s modified eagle medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS) (Lonza, Basel, Switzerland), 1% Penicillin-Streptomycin Solution (100 U/ml of penicillin and 0.1 mg/ml of Streptomycin) according our previous study¹⁸, in a humidified atmosphere with 5% CO₂ at 37 °C. The cells were plated into 96-well plates at a density of 2 × 10⁵ cells/well. Transfection was conducted when cells reached 60–70% confluence.
The cells only transfected with miRNA mimics or inhibitors (Ribobio) were for qRT-PCR assays and co-transfected with miRNA mimics or inhibitors reporter and plasmids were for Luciferase reporter assay. We used riboFECT^TM Transfection kit (Ribobio) to transfect 100 ng/well reporter plasmids (XPC 2229090-C, XPC 2229090-G or Blank controls), 50 nmol/L hsa-miR-589-5p mimics, 100 nmol/L hsa-miR-589-5p inhibitors, or 50 nmol/L miRNA mimics controls, 100 nmol/L inhibitor controls into the cells, respectively.

Zou X., Kang L., Yang M., Wu J, & Guan H. (2018). MicroRNA binding mediated Functional sequence variant in 3′-UTR of DNA repair Gene XPC in Age-related Cataract. Scientific Reports, 8, 15198.

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Transfection of Cell Lines for Functional Assays

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Human lens epithelial cell line (HLEPIC) and human embryonic kidney cell line (HEK-293T) and human hepatoblastoma cell line (HepG2) were purchased from American Type Culture Collection (ATCC; Rockville, MD, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, CA, USA) at 37 °C in a humidified incubator with 5% CO₂. Low passage cells were used in experiments 24 hours before transfection, cells were plated onto a 24-well plate at a density of 2×10⁵ cells/well. The transfection was conducted when cells reached 80% confluence.
HEK-293T and HepG2 cells were transiently co-transfected with reporter plasmids and miRNA mimics or inhibitors (Ribobio, Guangzhou, China). HEK-293T and HLEPIC cells were only transfected with miRNA mimics or inhibitors for qRT-PCR or Western blot assays. The miRNA inhibitors were chemically-modified and optimized complementary single stranded nucleic acids designed to specifically target the miRNA and knockdown individual miRNA molecules.

Rong H., Gu S., Zhang G., Kang L., Yang M., Zhang J., Shen X, & Guan H. (2017). MiR-2964a-5p binding site SNP regulates ATM expression contributing to age-related cataract risk. Oncotarget, 8(49), 84945-84957.

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Overexpression and Knockdown of Key Regulators

Cited 2 times

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For overexpression of circ-G004213, the full-length 339 bp cDNA of circ-G004213 was cloned into the vector pLCDH. The circ-G004213 and 2581-bp to 2600-bp fragments of PRPF39 were amplified from the cDNA of HepG2 cells and cloned into pmiRGLO (Promega Corporation) for luciferase analysis. All constructed plasmids were confirmed by sequencing. In addition, HepG2 cell lines with stable overexpression or knockout of PRPF39 were constructed with lentiviral vectors (24 (link),25 (link)). Short interfering (si)RNA, miRNA mimics or inhibitors were synthesized by Guangzhou RiboBio Co., Ltd. siRNA-circ-G004213, 5′-AGAUUCUUAGACUCCAGAU-3′; siRNA-NC (negative control), 5′-UUCUCCGAACGUGUCACGU-3′. HepG2 cells were seeded in a six-well plate, and 100 pM of siRNA-circ-G004213 was transfected into HepG2 cells using TurboFect transfection reagent (Thermo Fisher Scientific, Inc.). miRNA mimics or inhibitors (Beckman Coulter, Inc.) were diluted to a final concentration of 20 nM in serum-free DMEM (HyClone; Cytiva). For transient transfection, HepG2 and 293T cells were transfected with Lipofectamine^® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. There was an interval of 24 h between transfection and subsequent experiments.

Qin L., Zhan Z., Wei C., Li X., Zhang T, & Li J. (2021). Hsa-circRNA-G004213 promotes cisplatin sensitivity by regulating miR-513b-5p/PRPF39 in liver cancer. Molecular Medicine Reports, 23(6), 421.

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Plasmid and miRNA Transfection Protocol

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Transfection of plasmids was performed using Lipofectamine TM 3000 reagent (Invitrogen, USA) according to the manufacturer's instructions. Transfection of miRNA mimics or inhibitors (Ribobio, China) was performed using Lipofectamine RNAiMAX (Invitrogen, USA) at a nal concentration of 20 nM.

Peng J., Yu J., Liu J., Wei H., Song H., Shi S., Wang S., Guo D., Li Z., He S., Feng X., Li L., Zhao R., Liu Y., Liu Y., Li J., Zhang R., & Wang W. (2020). Absence of miR-378d Promoted The Malignant Phenotype of ESCC Through The AKT-β-Catenin and Hippo-p53 Signaling Pathway.

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