Samples of leaf tissue were collected from randomly selected plants, and the four fully expanded young leaves were taken for biochemical analysis. One part of the samples was used to perform the determinations in fresh tissue immediately, and the other samples were stored at –20 °C and then lyophilized for 72 h at −84 °C and 0.060 mbar in a freeze dryer (Labconco, FreeZone 2.5 L model, MO, USA). The fruit samples were collected and handled as described by López-Vargas et al. [42 (link)]. For this, uniformly sized fruits were collected at stage six (light red) of maturity, according to the color visual scale of USDA [56 ]. The fruits were selected after verifying that they were not physically damaged and that they were uniform, and were washed. Six fruits were used whole to perform the determinations in fresh tissue immediately, and six fruits were frozen at −20 °C and then lyophilized for 72 h at −84 °C and 0.060 mbar in a freeze dryer (Labconco, FreeZone 2.5 L model, MO, USA). The lyophilized samples were ground to a fine powder to perform the determinations in lyophilized tissue.
Freezone 2.5 l model
Manufactured by Labconco
Sourced in United States
The FreeZone 2.5 L model is a compact freeze dryer designed for laboratory use. It features a 2.5 liter stainless steel collector chamber and can reach temperatures as low as -50°C. The equipment is capable of freeze drying small sample volumes.
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Lab products found in correlation
3 protocols using freezone 2.5 l model
1
Leaf and Fruit Sample Preparation
2
Leaf and Fruit Sampling Protocol
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Random plants were selected, and four fully expanded young leaves were sampled. At harvest stage, 12 fruits of uniform size and red in the ripening stage 6 were selected according to the United States Department of Agriculture (USDA) scale [65 ]. Half of the sampled leaves and fruits were used for fresh determinations. The other half was stored at −20 °C and then lyophilized for 72 h at −84 °C and 0.060 mbar (Labconco, FreeZone 2.5 L model, Kansas City, MO, USA).
3
Effects of CNM Seed Priming on Tomato Growth
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After seed priming with CNMs for 24 h, a seed was placed in a 1 L polystyrene cup (L) with a mix of perlite substrate and peat moss in a 1:1 ratio. We considered a total of 10 cups for each treatment, where each vessel was a repeat. A directed irrigation system was used for irrigation, applying a Steiner solution (1961) at a 25% concentration to provide the necessary nutrients. The pH of the nutrient solution was adjusted with sulfuric acid (98%) to 6.5. The tomato plants were grown for 60 days in a chapel-type greenhouse, with a polycarbonate cover, an average PAR (photosynthetically active radiation) of 600 µmol m -2 s -1 , a temperature of 28 • C, and relative humidity of 60%.
Sixty days after planting, the plant height, stem diameter, and number of leaves were measured. In addition, the fresh and dry biomass of the aerial part and the root were determined.
For the determination of the biochemical compounds, samples of fresh foliar tissue were taken from fully expanded young leaves (third and fourth leaves), and these were placed in a freezer at -20 • C and then lyophilized for 72 h in freeze dryer (Labconco, FreeZone 2.5 L model, Kansas City, MO, USA). The lyophilized samples were ground to a fine powder to make the determinations.
Sixty days after planting, the plant height, stem diameter, and number of leaves were measured. In addition, the fresh and dry biomass of the aerial part and the root were determined.
For the determination of the biochemical compounds, samples of fresh foliar tissue were taken from fully expanded young leaves (third and fourth leaves), and these were placed in a freezer at -20 • C and then lyophilized for 72 h in freeze dryer (Labconco, FreeZone 2.5 L model, Kansas City, MO, USA). The lyophilized samples were ground to a fine powder to make the determinations.
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