Incucyte zoom analysis software

Manufactured by Sartorius

Sourced in United States

The Incucyte Zoom Analysis software is a real-time, automated live-cell imaging and analysis system. It provides quantitative insights into cellular processes by capturing and analyzing images of cells over time.

Automatically generated - may contain errors

Lab products found in correlation

Incucyte flr imaging system, by Sartorius (2 mentions) Incucyte zoom instrument, by Sartorius (1 mentions) Incucyte scratch wound cell migration and invasion system, by Sartorius (1 mentions) Mitomycin c, by Merck Group (1 mentions) Tototm 3 iodide, by Thermo Fisher Scientific (1 mentions) Incucyte system, by Sartorius (1 mentions) Countess automated cell counter, by Thermo Fisher Scientific (1 mentions) Incucyte platform, by Sartorius (1 mentions)

5 protocols using incucyte zoom analysis software

Quantifying Drug Response in Cell Growth Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells grown for 1 day in full medium were treated with the indicated drugs in 4 different concentrations (0.1, 1, 10 and 100 μM Fig 1B) along with the corresponding DMSO controls on the same plate. The growth of the cells was tracked by phase contrast imaging for 72h with 4 images per well taken every 2h using the Incucyte Zoom instrument (Essen BioScience) and the confluency estimated using the Incucyte Zoom Analysis software (Essen BioScience). The growth rate was estimated with a linear fit on the log-transformed confluency, and the IC50 was determined by fitting a sigmoid of the form:

\begin{matrix} V = \frac{1}{1 + exp (- log (C) + I C 50) \times S} \end{matrix}

to normalised growth rates (implemented in https://github.com/MathurinD/drugResistance). V is the growth rate relative to DMSO control, C is the concentration and the parameters IC50 and slope S are fitted. See S1 Table for the fitted parameters and S2 File for the raw data and analysis scripts, as well as S18 and S19 Figs for example images.

Dorel M., Klinger B., Mari T., Toedling J., Blanc E., Messerschmidt C., Nadler-Holly M., Ziehm M., Sieber A., Hertwig F., Beule D., Eggert A., Schulte J.H., Selbach M, & Blüthgen N. (2021). Neuroblastoma signalling models unveil combination therapies targeting feedback-mediated resistance. PLoS Computational Biology, 17(11), e1009515.

+ Open protocol

+ Expand

Measuring Cell Migration using Scratch Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Approximately 100,000 cells per well were seeded in 96-well ImageLock plates, yielding 90–100% confluency 24 hours after plating. To inhibit proliferation, cells were treated with 2 µg/ml mitomycin C (Sigma) for 1 hour and wounds generated using the Incucyte wound maker [19] . After rinsing with phosphate-buffered saline 3 times, cells were further cultured in the IncuCyte® Scratch Wound Cell Migration and Invasion System (Essen BioScience) and images were taken every hour during the 24 hour incubation. The relative percentage of wound area occupied by migrated cells was calculated relative to the original wound area using IncuCyte ZOOM analysis software (Essen BioScience).

Chen D., Cox J., Annam J., Weingart M., Essien G., Rathi K.S., Rokita J.L., Khurana P., Cuya S.M., Bosse K.R., Pilgrim A., Li D., Shields C., Laur O., Maris J.M, & Schnepp R.W. (2020). LIN28B promotes neuroblastoma metastasis and regulates PDZ binding kinase. Neoplasia (New York, N.Y.), 22(6), 231-241.

+ Open protocol

+ Expand

Quantifying Cell Proliferation and Death

Check if the same lab product or an alternative is used in the 5 most similar protocols

Control shLacZ JW23.3, shTyk2 #1 JW23.3, shTyk2 #2 JW23.3, and shTyk2 #3 JW23.3, were cultured in standard conditions with 2 µg/mL puromycin, then plated at 5000 cells/well in 96‐well plates. For proliferation assays, cells were plated in phenol‐red free DMEM with 10% FBS. Cells were imaged every hour for 48 hours using the IncuCyte FLR imaging system (Essen Bioscience, Ann Arbor, MI, USA) and analyzed for quantitation using IncuCyte ZOOM Analysis Software (Essen Bioscience, Ann Arbor, MI) Phase images were used to determine percent confluence and subsequent wells were normalized to initial confluence. For cell death assays, 50nM TOTO^TM‐3 iodide (ThermoFisher Scientific, USA) was added to the phenol‐red free media with reduced serum as a stress (5% FBS). Cells were imaged every hour for 48 hours using the IncuCyte FLR imaging system (Essen Bioscience, Ann Arbor, MI). For quantification of cell death, the TOTO^TM‐3 iodide fluorescence was normalized to the confluency factor calculated from the phase of each respective well.

Qin W., Godec A., Zhang X., Zhu C., Shao J., Tao Y., Bu X, & Hirbe A.C. (2019). TYK2 promotes malignant peripheral nerve sheath tumor progression through inhibition of cell death. Cancer Medicine, 8(11), 5232-5241.

+ Open protocol

+ Expand

Cell Proliferation and Clonogenic Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were counted using the Countess Automated Cell Counter (Invitrogen), plated in 96-well plates (~ 15,000 cells/well), and allowed to grow overnight. For proliferation assays, cells were plated in phenol-red free DMEM with 10% FBS. Cells were imaged every hour for 48 hours using the Incucyte FLR imaging system (Essen Bioscience, Ann Arbor, MI) and analyzed for quantitation using Incucyte ZOOM Analysis Software (Essen Biosciences). Phase images were used to determine percent confluence and subsequent wells were normalized to initial confluence. For clonogenic assays, control (shLacZ), and shTRIM23 Cell lines (1,2,3) were seeded at 200 cells/well in a 6 well dish (triplicates) in 3-mL DMEM medium. An initial image was taken 24 hours after plating (0 h) and allowed to grow for 2 weeks where a final image was taken (2 wk) using the IncuCyte system (Sartorius, Ann Arbor, MI). For quantification of colonies, using the image analysis software provided with the IncuCyte system, cell masks were calculated for each respective well to quantify confluency at each time point.

Godec A., Jayasinghe R., Chrisinger J.S., Prudner B., Ball T., Wang Y., Srihari D., Kaushal M., Dietz H., Zhang X., Pekmezci M., Dahiya S., Tao Y., Luo J., Van Tine B.A., Ding L., Gutmann D.H, & Hirbe A.C. (2019). Whole exome sequencing reveals the maintained polyclonal nature from primary to metastatic malignant peripheral nerve sheath tumor in two patients with NF1. Neuro-oncology Advances, 2(Suppl 1), i75-i84.

+ Open protocol

+ Expand

Real-time Cellular Confluence Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell seeding density was optimized to reach 60 to 80% confluence after 96h incubation. Real-time cell confluence monitoring was performed using the Incucyte platform (Essen BioScience) acquiring cell images every 2 h for 96 h. Cell confluence was determined at every time point for every well on the screening plates, using IncucyteZOOM analysis software.

Gaudeau A., Provost C.C., Dorval T., Walsh A., Hannus M., Perez F., Camonis J., Nery E.D., & Stéphan J. (2021). Cell-based siRNA screens highlight triple-negative breast cancer cell epigenetic vulnerability. International Journal of Scientific Reports, 7(4), 196-196.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!