Slc15a4−/− mice were treated with 20 mg kg−1 5-FU, and bone marrow cells were recovered 3 days later from the tibia and femur. Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 2% streptomycin/penicillin, 10 ng ml−1 IL-3 (Peprotech), 10 ng ml−1 thrombopoietin (Wako Pure Chemical) and 100 ng ml−1 SCF (Peprotech) for 2 days. The retrovirus carrying WT or an E465K mutation of human SLC15A4 was introduced twice into the bone marrow cells, and 2 days after transduction, the cells were cultured for 8 weeks with RPMI 1640 medium supplemented with 10 ng ml−1 IL-3 and selected with puromycin (2 μg ml−1). Myc-tagged human TFEB-WT, -S142A or -4RA (R245A, R246A, R247A, R248A) was introduced into the RBL2H3 or P815 cells.
Thrombopoietin
Manufactured by Fujifilm
Sourced in Canada
Thrombopoietin is a glycoprotein growth factor that stimulates the production and differentiation of megakaryocytes, the precursor cells of platelets. It plays a crucial role in regulating platelet production and maintaining normal platelet levels in the blood.
Automatically generated - may contain errors
Lab products found in correlation
Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions)
Stem cell factor (scf), by Thermo Fisher Scientific (1 mentions)
Stem cell factor (scf), by Fujifilm (1 mentions)
Vascular endothelial growth factor (vegf), by Fujifilm (1 mentions)
Primaria, by BD (1 mentions)
Flt 3 ligand, by Fujifilm (1 mentions)
Stemspam sfem, by STEMCELL (1 mentions)
2 protocols using thrombopoietin
1
Slc15a4 Knockout Bone Marrow Culture
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Slc15a4−/− mice were treated with 20 mg kg−1 5-FU, and bone marrow cells were recovered 3 days later from the tibia and femur. Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 2% streptomycin/penicillin, 10 ng ml−1 IL-3 (Peprotech), 10 ng ml−1 thrombopoietin (Wako Pure Chemical) and 100 ng ml−1 SCF (Peprotech) for 2 days. The retrovirus carrying WT or an E465K mutation of human SLC15A4 was introduced twice into the bone marrow cells, and 2 days after transduction, the cells were cultured for 8 weeks with RPMI 1640 medium supplemented with 10 ng ml−1 IL-3 and selected with puromycin (2 μg ml−1). Myc-tagged human TFEB-WT, -S142A or -4RA (R245A, R246A, R247A, R248A) was introduced into the RBL2H3 or P815 cells.
+ Expand
Slc15a4−/− mice were treated with 20 mg kg−1 5-FU, and bone marrow cells were recovered 3 days later from the tibia and femur. Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 2% streptomycin/penicillin, 10 ng ml−1 IL-3 (Peprotech), 10 ng ml−1 thrombopoietin (Wako Pure Chemical) and 100 ng ml−1 SCF (Peprotech) for 2 days. The retrovirus carrying WT or an E465K mutation of human SLC15A4 was introduced twice into the bone marrow cells, and 2 days after transduction, the cells were cultured for 8 weeks with RPMI 1640 medium supplemented with 10 ng ml−1 IL-3 and selected with puromycin (2 μg ml−1). Myc-tagged human TFEB-WT, -S142A or -4RA (R245A, R246A, R247A, R248A) was introduced into the RBL2H3 or P815 cells.
2
Expansion of CD34+ Hematopoietic Cells
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+ Expand
At first, 50,000 PB-CD34+ cells in 2 ml of medium were plated into each well of a six-well tissue culture dish (Primaria; BD Biosciences, San Diego, CA) and cultured in a suspension manner using serum-free culture medium (StemSpam SFEM; StemCell Technologies, Vancouver, Canada) containing 50 ng/ml VEGF (Wako, Richmond, VA), 100 ng/ml stem cell factor (Wako), 20 ng/ml interleukin-6 (Wako), 100 ng/ml Flt-3 ligand (Wako), and 20 ng/ml thrombopoietin (Wako) for 7 days, as described previously.22 (link),35–37
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