Acquity uplc csh c18 vanguard precolumn
The Acquity UPLC CSH C18 VanGuard precolumn is a component designed for use in ultra-performance liquid chromatography (UPLC) systems. It is intended to protect the primary analytical column from particulates and other contaminants, helping to extend the column's lifespan and maintain system performance.
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4 protocols using acquity uplc csh c18 vanguard precolumn
UPLC-QToF MS Lipid Analysis Protocol
LC-MS Analysis of Metabolite Profiles
Untargeted Lipidomics Analysis Protocol
Lipidomics Analysis by UPLC-QTOF-MS
Separation of lipids was performed on a Acquity UPLC CSH C18 column (100 × 2.1 mm, 1.7 μm, Waters) coupled to an Acquity UPLC CSH C18 VanGuard pre-column (5 × 2.1mm, 1.7 μm, Waters), operating at 55 °C. Injection volume of 4 µL sample.
The Acquity UPLC operated at a flow rate of 0.350 µL/min and the mobile phase consisted of acetonitrile—water (60:40; v/v) with 10 mM ammonium formate (solution A) and isopropanol-acetonitrile (90:10; v/v) with 10 mM ammonium formate (solution B). Initial conditions started with 40% B, and immediately a linear gradient from 40 to 100% B in 0 to 13 min, the eluent composition returned to the initial conditions in 0.1 min, and the column was equilibrated at the initial conditions for 3 min before the next injection, leading to a total run time of 16 min for each sample. For both positive and negative ionization modes, the same chromatographic conditions were used. Data collection and system control were performed using Waters MassLynx software (version 4.1, Waters Corporation, Milford, MA, USA).
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