Antibodies to BMP2, SYCP3, MVH, γHis2A.X and MCM2 were obtained from Abcam (Cambridge, MA). Alexa-conjugated secondary antibodies were obtained from Invitrogen (Carlsbad, CA). Plastic embedding medium was obtained from Electron Microscopy Sciences (Hatfield, Pa). Tissue-Tek Optimal Cutting Temperature (OCT) compound was obtained from Sacura Finetek USA Inc. (Torrance, CA). Phenol red-free DMEM, linolenic acid, BSA, and fine chemicals were purchased from Sigma Chemical Company (St. Louis, MO). LDN 193,189 was purchased from Sigma Chemical Co. (St. Louis, MO). DMH1 was purchased from Tocris Bioscience (Bristol, UK). Human holo-transferrin was obtained from BD pharmaceuticals (San Jose, CA). Quantitative RT-PCR primers and probes were synthesized by Sigma Chemical Company. RNeasy mini kit and Taq DNA polymerase were from Qiagen, Inc. (Valencia, CA). The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) kit was from Roche pharmaceuticals (Mannheim, Germany). All other molecular biology grade chemicals were obtained from Sigma Chemical Company or Fisher Scientific (Pittsburgh, PA).
Plastic embedding medium
Manufactured by Electron Microscopy Sciences
Sourced in Panama
Plastic embedding medium is a type of laboratory equipment used in specimen preparation for electron microscopy. It serves as a support matrix to embed and preserve the structure of biological or non-biological samples, allowing for thin sectioning and subsequent observation under an electron microscope.
Automatically generated - may contain errors
Lab products found in correlation
Taq dna polymerase, by Qiagen (2 mentions)
Rneasy mini kit, by Qiagen (2 mentions)
Linolenic acid, by Merck Group (1 mentions)
Phenol red free dmem, by Merck Group (1 mentions)
Quantitative rt pcr primers and probes, by Merck Group (1 mentions)
Sycp3, by Abcam (1 mentions)
Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Ldn193189, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling tunel kit, by Roche (1 mentions)
2 protocols using plastic embedding medium
1
Immunofluorescence Staining of Stem Cells
2
Ovarian Steroidogenesis Pathway Characterization
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