Agilent mass hunter acquisition software version b 04

Chromatographic analysis was performed on an Agilent 1290 LC instrument (Agilent Technologies, GmbH, Waldbronn, Germany) with a binary pump, an online degasser, an auto plate-sampler, and a thermostatically controlled column compartment. The separation was carried out on an Agilent C18 column (4.6×250 mm, 5 µm). A gradient mobile phase system of water (0.1% formic acid, eluent A) and ACN (0.1% formic acid, eluent B) was applied as follows: 79% A and 21% B (0 min), 79% A and 21% B (16 min), 70% A and 30% B (27 min), 55% A and 45% B (34 min), 25% A and 75% B (40 min), 100% B (46 min). The flow rate was set at 1 ml/min and the injected sample was 2 µl.
Detection was performed by a 6520 Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) with a dual electrospray ionization (ESI) source. The mass parameters for detection were optimized in the following: Drying gas flow rate, 10 l/min; drying gas temperature, 320°C; nebulizer, 35 psig; capillary, 3500 V; OCT RFV, 750 V; and fragmentor voltage, 120 V. The range m/z from 100 to 2,000 were recorded in the negative mode. Agilent MassHunter Acquisition Software version B.04.00 (Agilent Technologies) was applied to monitor the operations and data acquisitions.