Rabbit anti lpl polyclonal antibody sc 32885

Aβ_25–35, a toxic fragment of the full-length Aβ peptide, was purchased from American Peptide Company (131602-53-4, United States), which needs to be solubilized in sterile water and aggregated at 37°C for 7 days before use. The siRNA transfection reagent was purchased from Guangzhou RiboBio Company Limited (C10511-1, Guangzhou, China). The Simple ChIP Enzymatic Chromatin IP Kit was obtained from Cell Signaling Technology (9003S, BOS, USA), and rabbit anti-acetylation of histone 3 lysine 9 (Ace-H3K9) polyclonal antibody for ChIP was from Merck Millipore (06942, Darmstadt, Germany). The rabbit anti-PPARγ polyclonal antibody (D262458), rabbit anti-HDAC2 polyclonal antibody (D155199), and rabbit anti-HDAC3 polyclonal antibody (D260481) were purchased from Bio Basic Inc. (Canada); rabbit anti-LPL polyclonal antibody (sc-32885) and rabbit anti-β-actin polyclonal antibody (sc-130656) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Goat anti-rabbit immunoglobulin G (lgG) secondary antibody was from Shanghai Sangon Biotech Company Limited (D111018, Shanghai, China). Cell culture medium was purchased from American Hyclone Inc. (SH30023.01B, USA).

βOHB was purchased from Shanghai Yingxin Laboratory Equipment Co., Ltd. (Shanghai, China); rabbit anti-β-actin polyclonal antibody (sc-130656), rabbit anti-HDAC2 polyclonal antibody (sc-7899), rabbit anti-HDAC3 polyclonal antibody (sc-11417) and rabbit anti-LPL polyclonal antibody (sc-32885)were purchased from Santa Cruz Biotechnology (Santa Cruz, California); rabbit anti-acetylation of histone 3 lysine 9 (Ace-H3K9) polyclonal antibody (YK0006), rabbit anti-Ace-H4K12 polyclonal antibody (YK0013) and rabbit anti-Histone H3.1 polyclonal antibody (YK0009) were purchased from ImmunoWay Inc. (USA). Rat anti-mouse CD31 monoclonal antibody (550274) was obtained from BD Biosciences (USA). Goat anti-rabbit immunoglobulin G (lgG) secondary antibody FITC-donkey anti-rabbit IgG secondary antibody and CY3-donkey anti-rat lgG secondary antibody were from Shanghai Sangon Biotech Co., Ltd. (Shanghai, China). Cell culture medium was purchased from American Hyclone Inc. (USA). Aβ₂₅₋₃₅ was obtained from American Peptide Inc. (USA), which is a toxic fragment of the full-length Aβ peptide. Before use, Aβ₂₅₋₃₅ was solubilized in sterile water and aggregated at 37°C for 7 d. Immunohistochemistry kits were obtained from Beijing Zhongshan Biotechnology (Beijing, China).