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Cd3 ln10

Manufactured by Leica
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Sourced in United Kingdom

The CD3 (LN10) is a laboratory equipment product from Leica. It is designed for the detection and analysis of specific cell surface markers. The core function of the CD3 (LN10) is to facilitate the identification and characterization of T-cells in biological samples.

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Lab products found in correlation

Axio imager z2 microscope, by Zeiss (1 mentions) Axiovision software v4, by Zeiss (1 mentions) Shandon cytospin 4, by Thermo Fisher Scientific (1 mentions) Cd68 pgm 1, by Agilent Technologies (1 mentions)

Close spelling variants of this product

CD3 (clone LN10, (13 citations) Anti-CD3 (clone LN10, (8 citations) Anti-CD3/LN10 (2 citations)

2 protocols using cd3 ln10

1

Cytospin and Immunostaining Protocol

Cited 1 time
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Cytospin of FACS-purified cells was prepared as described (60 (link)) using Shandon Cytospin 4 (Thermo Scientific). Giemsa-Wright staining was performed using Advia S60 (Siemens) and imaged using Axioimager.Z2 microscope with Axiovision software v4.8 (Carl Zeiss, Germany). Human tonsil paraffin sections were immunostained with the antibodies: anti-AXL (MM0098-2N33, Abcam), CD123 (BR4MS, Leica Biosystems) and CD3 (LN10, Leica Biosystems) using a Ventana Benchmark XT instrument.
Villani A.C., Satija R., Reynolds G., Sarkizova S., Shekhar K., Fletcher J., Griesbeck M., Butler A., Zheng S., Lazo S., Jardine L., Dixon D., Stephenson E., Nilsson E., Grundberg I., McDonald D., Filby A., Li W., De Jager P.L., Rozenblatt-Rosen O., Lane A.A., Haniffa M., Regev A, & Hacohen N. (2017). Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes and progenitors. Science (New York, N.Y.), 356(6335), eaah4573.
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2

Multiplex Immunohistochemistry Protocol

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Multiplex immunohistochemistry (MP-IHC) with six antibodies and DAPI was performed, using the following reagents: CD4 (EPR6855, Abcam plc, Cambridge, UK), CD8 (C8/144B, Abcam plc, Cambridge, UK), CD20 (L26, Abcam plc, Cambridge, UK), CD3 (LN10, Leica Biosystems Inc., Vienna, Austria), CD45RO (UCHL1, Cell Signaling Technology Europe, B.V., Leiden, The Netherlands), CD68 (PG-M1, Dako Agilent Pathology Solutions, Agilent, Vienna, Austria). Tyramide-signal-amplification (TSA) kit (Akoya Biosciences, Marlborough, USA) technique was used for MP-IHC.
Smolle M.A., Herbsthofer L., Goda M., Granegger B., Brcic I., Bergovec M., Scheipl S., Prietl B., El-Heliebi A., Pichler M., Gerger A., Posch F., Tomberger M., López-García P., Feichtinger J., Baumgartner C., Leithner A., Liegl-Atzwanger B, & Szkandera J. (2021). Influence of tumor-infiltrating immune cells on local control rate, distant metastasis, and survival in patients with soft tissue sarcoma. Oncoimmunology, 10(1), 1896658.
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