Elisas

Manufactured by Merck Group

Sourced in Germany

ELISAs (Enzyme-Linked Immunosorbent Assays) are a widely used analytical technique for the detection and quantification of specific proteins, antibodies, or other analytes in various samples. ELISAs utilize the high specificity of antibody-antigen interactions to capture and detect target molecules, enabling sensitive and reliable measurements.

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Lab products found in correlation

Ultrasensitive insulin elisa, by Mercodia (1 mentions) Pge2 d4, by Cayman Chemical (1 mentions) Tsq quantum ultra triple quadrupole mass spectrometer, by Thermo Fisher Scientific (1 mentions) Total cholesterol and triglycerides, by Merck Group (1 mentions) Accela 1250 uhplc system, by Thermo Fisher Scientific (1 mentions) Infinity glucose oxidase, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Human LGALS3/Galectin‐3 ELISA Kit ChemiKine™ BDNF sandwich ELISA kit Human Ferritin ELISA Kit GLP-1 (active) ELISA Linco Human Adiponectin ELISA kit Rat/Mouse GIP (total) ELISA Milliplex ELISA kit Cell Proliferation ELISA Human PDGF-BB ELISA Kit BNP Elisa Kit

4 protocols using elisas

Adipokine and Glucose Measurement

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Plasma insulin, leptin, and adiponectin were measured using ELISAs (EMD Millipore, Temecula, CA, USA) according to the manufacturer’s instructions. Blood glucose was measured using a commercial glucometer (One Touch Ultra).

den Hartigh L.J., Wang S., Goodspeed L., Wietecha T., Houston B., Omer M., Ogimoto K., Subramanian S., Gowda G.A., O’Brien K.D., Kaiyala K.J., Morton G.J, & Chait A. (2017). Metabolically distinct weight loss by 10,12 CLA and caloric restriction highlight the importance of subcutaneous white adipose tissue for glucose homeostasis in mice. PLoS ONE, 12(2), e0172912.

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Insulin, Proinsulin, and GLP-1 Assays

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Laboratory tests of safety parameters were performed centrally at the Institute for Clinical Chemistry at Klinikum Schwabing (Medizet) using accredited methods. Insulin was measured at the Forschergruppe Diabetes e.V. using the Mercodia Ultrasensitive Insulin ELISA (Uppsala, Sweden). Proinsulin was measured with a quantitative ELISA (TecoMedical, Sissach, Switzerland), and glucose-dependent insulinotropic polypeptide (GIP) and GLP-1 were measured with ELISAs (Merck KGaA, Darmstadt, Germany) according to the manufacturer's instructions.

Hummel S., Beyerlein A., Pfirrmann M., Hofelich A., Much D., Hivner S., Bunk M., Herbst M., Peplow C., Walter M., Kohn D., Hummel N., Kratzsch J., Hummel M., Füchtenbusch M., Hasford J, & Ziegler A.G. (2018). Efficacy of vildagliptin for prevention of postpartum diabetes in women with a recent history of insulin-requiring gestational diabetes: A phase II, randomized, double-blind, placebo-controlled study. Molecular Metabolism, 9, 168-175.

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Serum Lipid and Eicosanoid Analysis

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Blood was collected during terminal procedures after fasting (5 h) and spun to isolate serum, then stored at −80 °C. Serum samples were subsequently analyzed for insulin and IL-10 levels using ELISAs (Millipore and R&D Systems, respectively) or total cholesterol and triglycerides (Sigma). For eicosanoid analysis, PGE₂ was extracted from serum in methanol and isolated by C18 solid-phase extraction chromatography. PGE₂-d₄ (Cayman Chemicals, Ann Arbor, MI, USA) was added to the experimental system as an internal standard. PGE₂ was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a TSQ Quantum Ultra triple quadrupole mass spectrometer coupled to an Accela 1250 UHPLC system (Thermo Scientific, Hemel Hempstead, UK) in negative ion mode with a C18 column and methanol-water gradient. Quantification was performed using the multiple reaction monitoring (MRM) mode using the transitions PGE₂ (351 → 271) and PGE₂-d₄ (355 → 193). The concentration of PGE₂ was determined by comparison to a calibration curve run in parallel.

Thompson D., Morrice N., Grant L., Le Sommer S., Ziegler K., Whitfield P., Mody N., Wilson H.M, & Delibegović M. (2017). Myeloid protein tyrosine phosphatase 1B (PTP1B) deficiency protects against atherosclerotic plaque formation in the ApoE−/− mouse model of atherosclerosis with alterations in IL10/AMPKα pathway. Molecular Metabolism, 6(8), 845-853.

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Comprehensive Metabolic Analysis Protocol

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Active GLP-1, total GIP, and total ghrelin were analyzed using ELISAs from Millipore (Darmstadt, Germany). Insulin was analyzed using an ELISA from Mercodia (Uppsala, Sweden), and glucose was analyzed using a commercially available kit (Infinity Glucose Oxidase) from Thermo Fisher Scientific (Lexington, MA, USA). Assays were performed according to the instructions provided by the manufacturers.

Lindqvist A., Shcherbina L., Fischer A.H, & Wierup N. (2017). Ghrelin Is a Regulator of Glucagon-Like Peptide 1 Secretion and Transcription in Mice. Frontiers in Endocrinology, 8, 135.

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