Zymosan z4250

Manufactured by Merck Group

Zymosan (Z4250) is a cell wall preparation derived from Saccharomyces cerevisiae, commonly known as baker's yeast. It is a heterogeneous mixture of polysaccharides, proteins, and other components. Zymosan is often used as a tool in biological research to study immune system responses and inflammatory processes.

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Lab products found in correlation

Tdm t3034, by Merck Group (1 mentions) Depleted zymosan, by InvivoGen (1 mentions) Anti syk syk01, by BioLegend (1 mentions) I a 1 e clone m5 114.15.2, by BioLegend (1 mentions) Luminol, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

2 protocols using zymosan z4250

Stimulating Macrophages with TDM and Zymosan

Cited 1 time

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TDM (T3034) and zymosan (Z4250) were purchased from Sigma-Aldrich. M. tuberculosis H37Ra and M. bovis BCG were obtained from Difco and Japan BCG Laboratory, respectively. ELISA kit for guinea pig TNF (DY5035) was from R&D Systems. For stimulation of reporter cells and peritoneal macrophages, TDM dissolved in chloroform:methanol (2∶1) at 1 mg/ml were diluted in isopropanol and added on 96-well plates at 20 µl/well, followed by evaporation of the solvent as previously described [6] (link).

Toyonaga K., Miyake Y, & Yamasaki S. (2014). Characterization of the Receptors for Mycobacterial Cord Factor in Guinea Pig. PLoS ONE, 9(2), e88747.

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Signaling Pathways in Immune Cells

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The following antibodies were purchased from Cell Signaling Technology, Inc.: pAKT S473 (9271), AKT (9272), p-ERK T202/Y204 (9106), ERK (9102), p-Syk Y525/526 (2710), p-PLCγ2 Y759 (3874), p-Src family Y416 (2101), Src (2110), p-Shp2 Y542 (3715), Shp2 (3397), p-P65 S536 (3033), and P65 (6956). Anti-Syk (Syk01) and anti-Nos2 (5C1B52) antibodies were from BioLegend. Antibodies to PLCγ2 (sc5283) were from Santa Cruz Biotechnology. Antibodies to Sts-1 and Sts-2 were previously described (17 (link), 54 (link)). Dectin-1 antibody (2A11) was from Bio-Rad, and flow cytometry antibodies to CD45 (clone 30-F11), CD11b (clone M1/70), CD11C (clone N418), F4/80 (clone BM8), Ly6g (clone 1A8), Ly6c (clone AL-21), and I-A/I-E (clone M5/114.15.2) were from BioLegend. Luminol, PMA, horseradish peroxidase (HRP) type VI (P8375), and zymosan (Z4250) were from Sigma-Aldrich. Particulate β-glucan, soluble β-glucan, and depleted zymosan were obtained from InvivoGen.

Frank D., Naseem S., Russo G.L., Li C., Parashar K., Konopka J.B, & Carpino N. (2018). Phagocytes from Mice Lacking the Sts Phosphatases Have an Enhanced Antifungal Response to Candida albicans. mBio, 9(4), e00782-18.

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