Anti mouse p21

The following antibodies were purchased from eBioscience (San Diego, CA, USA): anti-mouse CD3-APC (clone 145-2C11), anti-mouse CD117 (c-kit) APC (clone 2B8), anti-mouse Sca-1-PE (clone D7), anti-mouse CD34-FITC (clone RAM34), and anti-mouse Ki67-FITC (clone SolA15). The following antibodies were purchased from Biolegend (San Diego, CA, USA): Percp-conjugated streptavidin, anti-mouse B220-PE (clone RA3-6B2), anti-mouse CD11b-Percp (clone M1/70), anti-mouse Gr1-Percp (clone RB6-8C5), anti-mouse CD45.1-FITC (clone A20), anti-mouse CD45.2-PE (clone 104), biotin-conjugated anti-mouse Ter119 (clone TER119), anti-mouse B220 (clone RA3-6B2), anti-mouse Gr1 (clone RB6-8C5), anti-mouse CD11b (clone M1/70), anti-mouse CD4 (clone GK1.5), and anti-mouse CD8 (clone 53–6.7). The following antibodies were purchased from BD Bioscience (San Jose, CA, USA): anti-mouse annexin V-FITC and anti-mouse γ-H2AX-FITC. The following antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA): anti-mouse NRF2, anti-mouse BCL-XL, and anti-mouse BAK. Anti-mouse p21 and anti-mouse β-actin were purchased from Abcam (Cambridge, England). Anti-mouse 8-oxoguanine was purchased from Millipore (Boston, MA, USA).

As described previously [23 (link)], cells were incubated with the relative primary antibody overnight at 4 °C, followed by incubation with fluorescent secondary antibody for 2 h at room temperature. After final washes with phosphate-buffered saline (PBS), the coverslips were mounted using an antifade mounting solution containing 4,6-diamidino-2-phenylindole (DAPI; P36935, Invitrogen). Images were acquired on the confocal microscope (Carl Zeiss). Primary antibody:anti-rabbit p21 (catalog number 2947) from Cell Signaling Technology; anti-mouse p21 (catalog number ab80633) from Abcam for Immunofluorescence double-staining analysis; anti-rabbit NEDD4 (catalog number 2740) from Cell Signaling Technology.