Primary human epidermal keratinocytes

Manufactured by Lonza

Sourced in Switzerland

Primary human epidermal keratinocytes are a type of cell derived from the epidermis, the outermost layer of human skin. These cells are used in various research and development applications.

Automatically generated - may contain errors

Lab products found in correlation

Rat tail type 1 collagen solution, by Corning (1 mentions) Tryple express, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Bj 5ta human fibroblasts, by American Type Culture Collection (1 mentions) Kgm gold keratinocyte growth medium, by Lonza (1 mentions) Kgm gold single quot kit, by Lonza (1 mentions) Dulbecco modified eagle medium (dmem), by Lonza (1 mentions)

Close spelling variants of this product

Primary Human Keratinocytes ( (3 citations) Human epidermal keratinocytes (2 citations)

2 protocols using primary human epidermal keratinocytes

Characterization of Human Skin Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture reagents Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), 0.05% trypsin-EDTA and TrypLE™ Express were purchased from Gibco (USA). CnT-Prime epithelial proliferation medium (CnT-PR), CnT-Prime epithelial 3D airlift medium (CnT-PR-3D) and CnT-Prime Fibroblast Proliferation medium (CnT-PR-F) were bought from CELLnTEC (Switzerland). Primary human epidermal keratinocytes and KGM-Gold Keratinocyte Growth Medium (KGM) were purchased from Lonza (Switzerland). Rat tail collagen type I solution from Corning (USA) and lyophilized bovine collagen type I from Symatese (France) were used. Human dermal fibroblast (HDF/TERT164) cell line was purchased from Evercyte (Austria) while immortalized cell line N/TERT was a gift from Associate Professor Hao Li, Amy (National University of Singapore, Singapore).
Primary and secondary antibodies used for immunofluorescence staining are listed in Table 1.

Chai R.J., Wong W.L, & Beh C.W. (2023). Developing a bioink for single-step deposition and maturation of human epidermis. International Journal of Bioprinting, 9(4), 738.

+ Open protocol

+ Expand

Culturing Primary Human Keratinocytes and Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary human epidermal keratinocytes (Lonza, Basel, Switzerland) were cultured in KGM-Gold™ Keratinocyte Growth Medium supplemented with KGM-Gold SingleQuot Kit (Lonza, Basel, Switzerland). BJ-5ta human fibroblasts (ATCC, VA, USA) were cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 10% heat inactivated fatal bovine serum (Corning, NY, USA) penicillin (100 U/ml) (GIBCO, MA, USA) and streptomycin (0.1 mg/ml) (GIBCO, MA, USA)41 (link).

Some S., Sohn J.S., Kim J., Lee S.H., Lee S.C., Lee J., Shackery I., Kim S.K., Kim S.H., Choi N., Cho I.J., Jung H.I., Kang S, & Jun S.C. (2016). Graphene-Iodine Nanocomposites: Highly Potent Bacterial Inhibitors that are Bio-compatible with Human Cells. Scientific Reports, 6, 20015.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!