Model 5100

Manufactured by PerkinElmer

The Model 5100 is a versatile laboratory instrument designed for the analysis and measurement of various samples. It features advanced technology and provides accurate and reliable data for scientific research and testing applications.

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Lab products found in correlation

Fias 400, by PerkinElmer (1 mentions)

4 protocols using model 5100

Biochemical Parameters and Oxidative Stress

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Biochemical parameters were measured in the absence of infectious processes. Following a 12-h fast, 10 mL of blood was collected by venipuncture.
Plasma selenium levels were determined by hydride generation atomic absorption spectrometry (HITACHI brand, model Z-5000) coupled to a quartz cell (HGQTAAS).¹⁴ (link) Values were considered low or high when they were below the 2.5 percentile or above the 9.7 percentile, respectively, for age and gender.¹⁵ (link) Serum copper measurement were analyzed by atomic absorption spectrophotometry (PERKIN ELMER brand, model 5.100).¹⁶ (link) The reference values used were 70–140 μg/dL for males and 80–155 μg/dL for females.¹⁷ Serum and erythrocyte zinc concentrations were analyzed by atomic absorption spectrophotometry (PERKIN ELMER brand, model 5.100).¹⁶ (link) The reference values are 40–44 μg Zn/g Hb for erythrocyte zinc,¹⁸ 65–120 μg/dL for serum zinc in males, and 60–120 μg/dL for serum zinc in females.¹⁷ SOD activity in erythrocytes was measured by spectrophotometry and adequate values range from 4.7 to 5.7 U/mg of hemoglobin (Hb).¹⁹ (link)
The Advia Chemistry System 1650 (Bayer^®) analyzer and a turbidimetric assay were used to evaluate the level of C-reactive protein (CRP) in the subjects.

Pugliese C., Patin R.V., Palchetti C.Z., Claudio C.C., de Fátima Thomé Barbosa Gouvêa A., de Menezes Succi R.C., Amancio O.M., Cozzolino S.M, & Oliveira F.L. (2014). Assessment of antioxidants status and superoxide dismutase activity in HIV-infected children. The Brazilian Journal of Infectious Diseases, 18(5), 481-486.

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Quantification of Toxic Heavy Metals in Urine

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We measured the levels of Cd, As, and Hg in urine. The methods used for analyzing heavy metals are detailed in our previous research.^{[4 (link)]} Cd concentration was determined using a flameless atomic absorption spectrophotometer (Hitachi Model Z-8270), which was outfitted with a Zeeman graphite furnace. Urine samples were combined with nitric acid, diluted using diammonium hydrogen phosphate and 1% Triton X-100, and then mixed thoroughly. The detection limit for Cd was 0.01 µg/L. Total As concentration in urine was analyzed using an atomic absorption spectrometer (PerkinElmer Model 5100) that incorporated a hydride generation system (PerkinElmer FIAS-400). Each urine sample was mixed with HCl, ascorbic acid, and potassium iodide (2:2:1:1); left to incubate for an hour; and then diluted with 10% HCl. The reducing agents used were 0.2% sodium borohydride and 0.5% sodium hydroxide. The mobile phase consisted of 3% HCl, with argon utilized as the carrier gas. The detection limit of the method was 0.2 µg/L. Urine Hg concentration was analyzed using the gold amalgam method with a direct Hg analyzer. After placing 100 µL of well-mixed urine in the sample container, the analysis was conducted immediately.

Choi S.H., Choi K.H., Won J.U, & Kim H. (2023). Impact of multi-heavy metal exposure on renal damage indicators in Korea: An analysis using Bayesian Kernel Machine Regression. Medicine, 102(41), e35001.

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Quantitative Analysis of Macro-Nutrients

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For measurement of macro elements, 0.3g from each sample of dried leaf powder was wet digested with a mixture of concentrated sulphuric and perchloric acids (Peterburgski, 1968) after digestion the cleared solution was quantitavely transferred into 50 ml measuring flask with distilled water and kept for the determinations. Measuring total nitrogen: it was done by using the Micro Kjeldahl apparatus according to Jones et al. (1991) . Total phosphorus content: it was determined spectrophotometrically according to Jackson (1973) . Na + , K + and Ca +2 were extracted according to Gavlak et al., (1994) . Leaf fine dry powder (0.2 g) was microwave digested with nitric/hydrogen peroxide, filtered through qualitative filter paper and used for Na + , K + and Ca +2 determinations by flame photometry (Gustav, 1961) . For estimation of Mg +2 , the method of microwave digestion was used. The concentration of Mg +2 was analyzed by electrothermal atomic absorption spectrometry, Perkin Elmer Model 5100 according to Kumpulainen et al., (1983) . The concentration of the elements was calculated on a dry weight basis.

El-Dengawy E., El-Abbasy U., & El-Gobba M.H. (2021). Influence of Nano-silicon Treatment on Growth Behavior of ‘Sukkary’ and ‘Gahrawy’ Mango Root-stocks Under Salinity Stress. Journal of Plant Production, 12(1), 49-61.

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Determination of Leaf Nutrient Concentrations

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For determination of N, P, K, and Na concentrations, mature leaf samples were oven-dried at 70 o C to a constant weight and were finely ground, 0.2 g of dried powder from each sample was digested wetly according to the method stated (Jackson, 1973) .
Estimation of total nitrogen was done by using the Micro Kjeldahl device according to Jones et al. (1991) . Total phosphorus was determined spectrophotometrically according to Jackson (1973) . Determination of total potassium and Sodium was done by using a Gallen Kamp flame photometer according to (Jackson, 1973) . The concentration of the elements was calculated as percentages on a dry weight basis.
For estimation of Ca 2+ and Mg 2+ , the method of microwave digestion was used. The concentration of the elements Ca 2+ and Mg 2+ was analyzed by electrothermal atomic absorption spectrometry, Perkin Elmer Model 5100 according to Kumpulainen et al., (1983) .

Doaa H., & Shalan A. (2020). Inducing Salinity Tolerance in Mango (Mangifera indica L.) Cv. “El-Gahrawey” by Sodium Silicate Pentahydrate and Glycine Betaine تعزیز تحمل شتلات المانجو صنف ̋ الجحراوى ̏ للملوحة بواسطة سلیکات الصودیوم والجلیسین بیتین. Journal of Plant Production, 11(6), 541-549.

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