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Pmirglo double luciferase mirna target expression vector

Manufactured by Promega
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Sourced in United States

The PmirGLO Double Luciferase miRNA Target Expression Vector is a tool designed for the study of microRNA (miRNA) target interactions. It contains two luciferase reporter genes, one for the miRNA target and another for normalization purposes. This vector enables the evaluation of the effect of miRNA binding on the expression of a target gene.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay, by Promega (1 mentions)

Close spelling variants of this product

PmirGLO luciferase miRNA Target Expression vector PmirGLO miRNA target expression vector PmirGLO luciferase Target Expression Vector PmirGlo luciferase expression vector PmirGLO luciferase vector PmirGLO expression vector PmirGLO vector PmirGLO

2 protocols using pmirglo double luciferase mirna target expression vector

1

TRAF6 miR-361-3p Binding Validation

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The TRAF6 fragments containing the presumed or mutant binding site of miR-361-3p were amplified by PCR and cloned into pmirGLO double luciferase miRNA target expression vector (Promega, USA) to obtain TRAF6-Wild-Type (TRAF6-WT) and TRAF6-Mutated-Type (TRAF6-MUT). MM1S and U266 cells were co-transfected with TRAF6-WT or TRAF6-MUT and blank or miR-361-3p mimic using Lipofectamine 2000 (Life Technologies, USA). The luciferase activity was detected by performing dual-luciferase reporter assay (Promega) 48 h after the cell transfection.
Fan Z., Wu Z, & Yang B. (2020). The Effect of miR-361-3p Targeting TRAF6 on Apoptosis of Multiple Myeloma Cells. Journal of Microbiology and Biotechnology, 31(2), 197-206.
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2

lncRNA004787 Binding Site Analysis

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According to the manufacturer’s instructions, the wild-type (wt) or mutant (mut) lncRNA004787 binding site was synthesized, and the pmiR-GLO double luciferase miRNA target expression vector (Promega, USA) was inserted after annealing. The oligonucleotides for annealing were listed in the supplementary information. The double-luciferase-reporter-gene-detection system (Vazyme, Nanjing, China) was adopted for double luciferase detection. Plasmids BACE1-miR107-mut, BACE1-miR107-wt and no-load pmiR-GLO were purchased from Suzhou Gemma Biotechnology Co., Ltd. (Suzhou, China).
Yao M., Xu C., Shen H., Liu T., Wang X., Shao C, & Shao S. (2020). The regulatory role of miR-107 in Coxsackie B3 virus replication. Aging (Albany NY), 12(14), 14467-14479.
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