Mcf10a human breast epithelial cells

Manufactured by American Type Culture Collection

Sourced in United States

MCF10A human breast epithelial cells are a non-tumorigenic, immortalized cell line derived from human breast tissue. These cells serve as a model for studying normal breast epithelial cell biology.

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Lab products found in correlation

Mdck 2 canine kidney epithelial cells, by American Type Culture Collection (3 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (3 mentions) Penicillin streptomycin, by Merck Group (2 mentions) Penicillin, by Atlanta Biologicals (1 mentions) Horse serum, by Atlanta Biologicals (1 mentions) Mda mb 453, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Skbr3, by American Type Culture Collection (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Hydrocortisone, by Merck Group (1 mentions)

Close spelling variants of this product

MCF10A cells (180 citations) MCF10A breast epithelial cells (2 citations)

6 protocols using mcf10a human breast epithelial cells

Cell Line Characterization and Maintenance

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No cell lines used in this study were found in the database of commonly misidentified cell lines maintained by the ICLAD and NCBI. MCF10A human breast epithelial cells and MDCK II canine kidney epithelial cells were purchased from ATCC and were maintained as previously described^{11 (link),41 (link)}. Cell lines were used for no more than twelve passages and were tested periodically for mycoplasma contamination (Lonzo MycoAlert). The cell lines were not authenticated. MDCKII lines expressing control shLuc, shLKB1 clones 11 and 14, and shE-cadherin were generous gifts from Dr. Michael Sebbagh^{17 (link)}. MDCKII cells were maintained in DMEM (4g/L D-glucose with L-Glutamine) with 10% FBS (Atlanta Biologicals) and 1× Penicillin/ Streptomycin (Sigma). These lines were chosen for they are both non-tumorigenic epithelial lines that form strong cell-cell adhesions which have been characterized by our laboratory and others^{11 (link),41 (link)-43 (link)}. 293GPG cells are a virus-producing cell line that are a derivative of 293T cells and were maintained as described previously^{11 (link)}.

Bays J.L., Campbell H.K., Heidema C., Sebbagh M, & DeMali K.A. (2017). Linking E-cadherin mechanotransduction to cell metabolism through force mediated activation of AMPK. Nature cell biology, 19(6), 724-731.

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Cell Line Characterization and Maintenance

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Characterization of Epithelial Cell Lines

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MCF-10A human breast epithelial cells and MDCKII canine kidney epithelial cells were purchased from ATCC and were maintained as previously described^{11 (link), 12 (link), 41 (link)}. All cell lines and their derivatives were used for no more than 12 passages and periodically checked for mycoplasma contamination. shGLUT1–1, shGLUT1–2, scrGLUT1, shGLUT3–1, shGLUT3–2, scrGLUT3 and scrANKG expressing cells were selected and maintained in puromycin (2μg/mL). shANKG cells were selected and cultured in G418. shEcad/WT and shEcad/PolyA were selected and maintained in blasticidin (3μg/mL)^{26 (link)}. shCtrl, shNMIIA and shNMIIB expressing cells were maintained in media containing geneticin (5 μg/mL) and puromycin (2.5 μg/mL). The 293GPG and 293FT (Invitrogen) cells are virus-producing cell lines that are a derivative of 293T cells. The 293 GPG were maintained as described previously^{8 (link)} and the 293FT were maintained in DMEM supplemented with 10% heat-inactivated FBS, 0.1mM MEM Non-essential Amino Acids (NEAA), 6mM L-glutamine, 1mM MEM Sodium Pyruvate, 1% Pen-Strep. MDCKII cell lines expressing shEcad were previously described^{18 (link)}.

Salvi A.M., Bays J.L., Mackin S.R., Mege R.M, & DeMali K.A. (2021). Ankyrin G Organizes Membrane Components to Promote Coupling of Cell Mechanics and Glucose Uptake. Nature cell biology, 23(5), 457-466.

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Culturing Breast Cell Lines

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MCF10A human breast epithelial cells (ATCC, Manassas, VA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM)/Ham’s F-12 (50/50) medium supplemented with 5% horse serum (Atlanta Biologicals, Flowery Branch, GA), insulin (10 μg mL⁻¹), epidermal growth factor (20 ng mL⁻¹), cholera toxin (100 ng mL⁻¹), hydrocortisone (0.5 μg mL⁻¹), and 1% antibiotics (penicillin & streptomycin) and antimycotics (amphotericin B) solution (Mediatech, Manassas, VA). SkBr3, MDA-MB-453, MCF-7, MDA-MB-231 and Hs 578T human breast cancer cells (ATCC) were routinely maintained in complete Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 9% fetal bovine serum (Atlanta Biologicals) and 1% penicillin and streptomycin solution. All cell culture media were purchased from Mediatech (Manassas, VA). Cells were cultured at 37°C in a humidified 5% CO₂ incubator.

Palasuberniam P., Kraus D., Mansi M., Braun A., Howley R., Myers K.A, & Chen B. (2019). Ferrochelatase Deficiency Abrogated the Enhancement of Aminolevulinic Acid-mediated Protoporphyrin IX by Iron Chelator Deferoxamine. Photochemistry and photobiology, 95(4), 1052-1059.

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Cell Culture Maintenance Protocols

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CCD18 Co normal colon and MCF-7 breast adenocarcinoma cells were maintained in ATCC-formulated Eagle's Minimum Essential Medium and HT29 colon carcinoma cells were maintained in McCoy's 5a Medium Modified supplemented with 10% foetal bovine serum (Atlas; Fort Collins, CO, USA), 10mM HEPES solution, 100 mM L-glutamine penicillin-streptomycin solution, 3 g/L glucose, and 1.5 g/L of sodium bicarbonate. MCF-10A human breast epithelial cells were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s Modified Eagle’s Medium/nutrient mixture F-12 (Mediatech, Herndon, VA, USA) supplemented with hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 5% (v/v) horse serum (Invitrogen, Carlsbad, CA, USA) cholera toxin (Calbiochem, BD Biosciences, La Jolla, CA, USA) and penicillin-streptomycin antibiotics (Mediatech, Herndon, VA, USA). For the apoptosis studies and certain cell viability assays, MCF-7 cells were maintained in RPMI medium supplemented with 10% FBS and penicillin and streptomycin antibiotics. All cells were maintained at 37°C in humidified air containing 5% CO₂.

Badal S.A., Valenzuela M.M., Zylstra D., Huang G., Vendantam P., Francis S., Quitugua A., Amis L.H., Davis W., Tzeng T.R., Jacobs H., Gangemi D.J., Raner G., Rowland L., Wooten J., Campbell P., Brantley E, & Delgoda R. (2017). Glaucarubulone glucoside from Castela macrophylla suppresses MCF-7 breast cancer cell growth and attenuates benzo[a]pyrene-mediated CYP1A gene induction. Journal of applied toxicology : JAT, 37(7), 873-883.

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Culture of MCF-10A Breast Cells

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MCF-10A human breast epithelial cells were purchased from American Type Culture Collection (ATCC, Manassas, VA). The cells were cultured in a humidified incubator at 37 °C under 5% CO₂ atmospheric conditions in RPMI media supplemented with 10 μg/ml insulin, 20 ng/ml epidermal growth factor, 10 mg/ml hydrocortisone, 5% horse serum and 1% penicillin-streptomycin (10,000 U/ml). Experiments were conducted within 25 passages.

Jain A., Samykutty A., Jackson C., Browning D., Bollag W.B., Thangaraju M., Takahashi S, & Singh S.R. (2015). Curcumin inhibits PhIP induced cytotoxicity in breast epithelial cells through multiple molecular targets. Cancer letters, 365(1), 122-131.

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