Anti gapdh g9295

RPMI medium, fetal bone serum (FBS), penicillin-streptomycin, L-glutamine, Opti-MEM and Lipofectamine™ 2000 were products of Invitrogen (Carlsbad, CA, USA). LPS derived from Pseudomonas aeruginosa, propidium iodide (PI), PMA and DMSO were purchased from Sigma Aldrich (St. Louis, MO, USA). C-176 (STING inhibitor) was bought from Selleck (Houston, TX, USA) and BAY11-7082 (NF-κB inhibitor) was bought from MedChemExpress (New Jersey, USA). 2’, 3’-cGAMP (STING agonist) and CpG ODN 2006 were bought from Invivogen (San Diego, CA, USA). Primary antibodies: anti-caspase-1 (AG-20B-0048) from Adipogen (San Diego, CA, USA); anti-caspase-4 (4450S), anti-GSDMD (97558S), anti-NF-κB p65 (8242S) and anti-P-NF-κB p65 (3033S) from Cell Signaling Technology (Beverly, MA, USA); another anti-caspase-4 (ab22687) from Abcam (Cambridge, MA, USA); anti-caspase-5 (M060-3) from Medical & Biological Laboratories(Nagoya, Japan); anti-β-actin (A1978) and anti-GAPDH(G9295) from Sigma Aldrich (St Louis, MO, USA).

Antibodies against Flag (F3165) (1:2000) and monoclonal mouse anti‐GAPDH (G9295) (1:5000) were purchased from Sigma (St Louis, MO, USA). Anti‐P21 (sc‐6246 Santa Cruz), anti‐P16^ink4a (sc‐166760 Santa Cruz), anti‐P16^ink4a (abs130164 Absin) and anti‐MYSM1 (Abcam, ab‐193081) (1:100) were purchased from the indicated manufacturers. Anti‐γ‐h2ax was purchased from Abcam.
Lipofectamine 2000, normal rabbit immunoglobulin G (IgG), and normal mouse IgG were purchased from Invitrogen (Carlsbad, CA, USA). Etoposide, and doxorubicin were purchased from Sigma (St Louis, MO, USA).

Triciribin and mevastatin were purchased from Selleckchem (Houston, Texas). Triciribine phosphate, U0126, actinomycin D (Act D), (±)-mevalonolactone, paraformaldehyde and saponin were from Sigma-Aldrich (St. Louis, Missouri). Antibodies against LDLR (3839; for Western blot analysis and immunofluorescence microscopy) and β-actin (ab8227) were obtained from BioVision (Milpitas, California) and Abcam (Cambridge, UK), respectively. Anti-LDLR (61087; for detection of cell-surface LDLR by flow cytometry) was obtained from Progen (Heidelberg, Germany). Anti-GAPDH (G9295) was from Sigma-Aldrich. Anti-β-tubulin (T9154-05G) was purchased from USBiological (Swampscott, Massachusetts).