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Vimentin antibody

Manufactured by Agilent Technologies
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The Vimentin antibody is a laboratory reagent used in various research applications. It is a protein that specifically binds to the intermediate filament protein Vimentin, which is commonly used as a marker for mesenchymal cells. The Vimentin antibody can be utilized in techniques such as immunohistochemistry, Western blotting, and flow cytometry to detect and analyze the expression of Vimentin in biological samples.

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Lab products found in correlation

Dmrxa2 microscope, by Leica (1 mentions) Rabbit e cadherin antibody, by Cell Signaling Technology (1 mentions) Cantharidin, by Merck Group (1 mentions) Lopac1280, by Merck Group (1 mentions) Type 1 collagen, by BD (1 mentions) Fibronectin, by BD (1 mentions)

Close spelling variants of this product

Mouse anti-vimentin monoclonal antibody Anti-vimentin antibody Anti-vimentin monoclonal antibody Mouseanti-vimentin monoclonal antibody (clone V9, Mouse anti-human vimentin antibody Anti-vimentin antibody (clone V9; Vimentin

2 protocols using vimentin antibody

1

Immunofluorescence Detection of Cell Markers

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To detect actin, E-cadherin and vimentin in MCF10A, cells were fixed with 4% paraformaldehyde for 15min and permeabilized with 0.1% Triton X-100 in PBS before incubation with rabbit E-cadherin antibody (Cell Signaling) followed by alexa 555-conjugated anti-rabbit (Cell Signaling), vimentin antibody (Dako) followed by alexa 555-conjugated anti-mouse (Cell Signaling) or rhodamine-phalloïdin (Interchim, Montluçon, France). The slides were washed, mounted and viewed using an automated LEICA DMRXA2 microscope.
Ruff M., Leyme A., Le Cann F., Bonnier D., Le Seyec J., Chesnel F., Fattet L., Rimokh R., Baffet G, & Théret N. (2015). The Disintegrin and Metalloprotease ADAM12 Is Associated with TGF-β-Induced Epithelial to Mesenchymal Transition. PLoS ONE, 10(9), e0139179.
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2

Small Molecule Screening Library Protocol

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The LOPAC1280 was obtained from Sigma-Aldrich (1,280 pharmacologically active small molecules including marketed drugs and pharmaceutically relevant structures annotated with biological activities). The Prestwick Chemical Library (1,140 small molecule drugs, 100% approved by Food and Drug Administration (FDA), European Medicines Agency (EMEA) and other agencies) was purchased from Prestwick Chemical. The active compounds were re-purchased from independent sources. Alexidine dihydrochloride (CAS 171530-6), beta-escin (CAS 6805-41-0) and cantharidin (CAS 56-25-7) were from Sigma-Aldrich, and sanguinarine (CAS 5578-73-4), prochlorperazine dimaleate (CAS 84-02-6) and tomatine (CAS 17406-45-0) were from Tocris Bioscience. CellTracker Green (CMFDA), Red (CMPTX) and blue (CMAC) were purchased from Life Technologies. The MMP inhibitor, GM6001 (EMD Bioscience), vimentin antibody (1:600 dilution, clone M0725, Dako Cytomation), cytokeratin 8 antibody (1:500 dilution, clone Ks8.7, Santa Cruz Biotechnology), collagen type I and fibronectin were purchased from BD Biosciences. The Arg–Gly–Asp and Arg–Ala–Asp peptides were from Sigma-Aldrich.
Kenny H.A., Lal-Nag M., White E.A., Shen M., Chiang C.Y., Mitra A.K., Zhang Y., Curtis M., Schryver E.M., Bettis S., Jadhav A., Boxer M.B., Li Z., Ferrer M, & Lengyel E. (2015). Quantitative high throughput screening using a primary human three-dimensional organotypic culture predicts in vivo efficacy. Nature communications, 6, 6220.
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