Image Studio 4.0 analytical software (LI-COR Biosciences) was used to measure the near-infrared fluorescence value for each target protein band of interest. For each subject, the mean of duplicate lanes for each protein was normalized to intralane VCP. Data were analyzed using Prism 6.04 software (GraphPad, La Jolla, CA). The D'Agostino-Pearson test was used to test for normality of distribution for each dependent variable. For experiments using human subjects, normally distributed data were analyzed using two-tailed paired Student’s t-tests, and for data not normally distributed, Wilcoxon matched-pair signed-rank tests were used. Data from rats were normally distributed and analyzed using two-tailed unpaired Student’s t-test. For all statistical analyses, α = 0.05.
Image studio 4.0 analytical software
Manufactured by LI COR
Sourced in United States
Image Studio 4.0 is an analytical software designed for image acquisition, processing, and analysis. It provides tools for quantifying and visualizing data from a variety of imaging platforms, including western blots, gels, and arrays. The software offers features for managing image files, performing basic image adjustments, and generating reports.
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Lab products found in correlation
Prism 6, by GraphPad (2 mentions)
Statistica 7, by StatSoft (1 mentions)
β actin mouse mab, by Cell Signaling Technology (1 mentions)
β actin, by Thermo Fisher Scientific (1 mentions)
Pparγ, by Cell Signaling Technology (1 mentions)
Fatty acid synthase, by Cell Signaling Technology (1 mentions)
Irdye 680, by LI COR (1 mentions)
Irdye 800, by LI COR (1 mentions)
3 protocols using image studio 4.0 analytical software
1
Quantitative Fluorescence Analysis of Proteins
2
Protein Expression Analysis in Schizophrenia
3
Protein Extraction and Immunoblotting Protocol
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Tissue was lysed with RIPA lysis buffer (50 mM Tris, pH 8, 150 mM NaCl, 1% Nonidet P-40, 0.1% SDS, 0.5% sodium deoxycholate), 0.5 mM PMSF and 1× protease inhibitor (10 µL/mL). Cellular debris was removed by centrifugation at 15,000 rpm for 15 min at 4 °C. Protein content of the clarified lysate was determined using bicinchoninic acid (BCA) reagents from Thermo Scientific (Rockford, IL, USA). Isolated proteins were denatured in SDS gel buffer, separated by SDS-PAGE, and immunoblotted using appropriate primary antibodies. Goat anti-rabbit or anti-mouse IRDye 680 (Cat# 926-68071) or IRDye 800 (Cat# 926-32210) secondary antibodies from LiCor were used for detection and quantitation of immunoblots. Membranes were imaged using a LiCor Odyssey scanner, and blots were analyzed by Image Studio 4.0 analytical software (LiCor, Lincoln, NE, USA) as previously described [61 (link),62 (link)]. Primary antibodies included PPARγ (Cat#2435), Fatty-Acid Synthase (Cat#3180), SCD1 (Cat#2438S), β-actin Mouse mAb (Cat#3700S) from Cell Signaling Technologies (Danvers, MA, USA) and β-actin (Cat#PA5-59497) from Invitrogen (Carlsbad, CA, USA).
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