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Epcam epithelium

Manufactured by BD
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The EpCAM+ epithelium is a lab equipment product that is used to isolate and enrich epithelial cells expressing the EpCAM (Epithelial Cell Adhesion Molecule) marker. It provides a means to selectively capture and extract epithelial cells from complex samples for further analysis or downstream applications.

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Lab products found in correlation

Calcein red orange am, by Thermo Fisher Scientific (2 mentions) Aria 2, by BD (2 mentions)

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EpCAM (37 citations)

2 protocols using epcam epithelium

1

Quantitative Organoid Proliferation and Invasion

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary organoids of the appropriate genotypes were recovered from collagen gel by collagenase IV digestion yielding a single cell suspension as described above at 10 days following viral infection. The cell suspension was FACS sorted (Aria II, BD) for EpCAM+ epithelium (BD Biosciences) and reseeded to collagen in 96-well air-liquid interface culture (BD FluoroBlok) at 10,000 cells per well, n=12 wells/genotype. Calcein red-orange, AM (Invitrogen, Molecular Probes) was used to stain cells at day 14 following replating (following the manufacturer’s instruction). The plate was analyzed by Flexstation II 384 using 577 nm excitation and 590 nm emission filters, respectively with the sum of top and bottom reads to quantitate proliferation and the bottom read only for invasion.
Li X., Nadauld L., Ootani A., Corney D.C., Pai R.K., Gevaert O., Cantrell M.A., Rack P.G., Neal J.T., Chan C.W., Yeung T., Gong X., Yuan J., Wilhelmy J., Robine S., Attardi L.D., Plevritis S.K., Hung K.E., Chen C.Z., Ji H.P, & Kuo C.J. (2014). Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture. Nature medicine, 20(7), 769-777.
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2

Quantitative Organoid Proliferation and Invasion

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary organoids of the appropriate genotypes were recovered from collagen gel by collagenase IV digestion yielding a single cell suspension as described above at 10 days following viral infection. The cell suspension was FACS sorted (Aria II, BD) for EpCAM+ epithelium (BD Biosciences) and reseeded to collagen in 96-well air-liquid interface culture (BD FluoroBlok) at 10,000 cells per well, n=12 wells/genotype. Calcein red-orange, AM (Invitrogen, Molecular Probes) was used to stain cells at day 14 following replating (following the manufacturer’s instruction). The plate was analyzed by Flexstation II 384 using 577 nm excitation and 590 nm emission filters, respectively with the sum of top and bottom reads to quantitate proliferation and the bottom read only for invasion.
Li X., Nadauld L., Ootani A., Corney D.C., Pai R.K., Gevaert O., Cantrell M.A., Rack P.G., Neal J.T., Chan C.W., Yeung T., Gong X., Yuan J., Wilhelmy J., Robine S., Attardi L.D., Plevritis S.K., Hung K.E., Chen C.Z., Ji H.P, & Kuo C.J. (2014). Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture. Nature medicine, 20(7), 769-777.
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