8 multi quartz micro cell array

The tRNA (2.5 μg) was dissolved in degassed buffer containing 20 mM HEPES-KOH (pH 7.5), 150 mM NaCl, and 6 mM MgCl₂, and incubated at 75 °C for 5 min, followed by cooling at room temperature for annealing. A UV–Vis spectrophotometer (V-630; JASCO) equipped with an 8 multi-quartz micro cell array (path length: 10 mm; JASCO) was used to monitor the hyperchromicity of tRNA. The temperature gradient was as follows: 25 °C for 30 s, and then ramped at 5 °C/min to 40 °C and held for 5 min, and ramped at 0.5 °C/min to 90 °C.

Twenty-five picomoles of tRNA mixtures were dissolved in degassed 20 mM HEPES–KOH (pH 7.5) buffer containing 100 mM NaCl and 1 mM MgCl₂ and were incubated at 80°C for 5 min, followed by cooling at room temperature for annealing. A UV-Vis spectrophotometer (V-630, JASCO) equipped with an 8 multi-quartz micro cell array (path length: 10 mm, JASCO) was used to monitor the hyperchromicity. The temperature gradient was as follows: 25°C for 30 sec, ramped at 5°C/min to 40°C and held for 5 min and after that ramped at 0.5°C/min to 105°C. The measurement was performed in triplicate for each sample.