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2 protocols using mouse anti acetylated α tubulin clone 6 11b 1

1

Comprehensive Antibody Panel for Cell Analysis

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The following primary antibodies were used: mouse anti-acetylated-α-tubulin clone 6-11B-1 and mouse anti-FLAG clone M2 (Sigma-Aldrich Co. Ltd.); rabbit-anti-γ-tubulin, rabbit anti-calbindin and mouse anti-β actin clone AC-15 (Abcam Ltd.); mouse anti-activated β-catenin (anti-ABC, clone 8E7) and mouse anti-Ki67 (Merck Millipore Inc.); rabbit mAb anti-phospho-histone H3(Ser10) clone D2C8 and rabbit anti-β-catenin (Cell Signaling Technology Inc.); and rabbit anti-HOXB5 antibody (Insight Biotechnology). Mouse anti-PAX6 was deposited to the Developmental Studies Hybridoma Bank by A. Kawakami (DSHB Hybridoma Product PAX6). Secondary antibodies were Alexa-Fluor 350-, Alexa-Fluor 488-, Alexa-Fluor 568- and Alexa-Fluor 594-conjugated goat anti-mouse IgG and goat anti-rabbit IgG (Molecular Probes Inc.).
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2

Immunoblotting and Immunofluorescence Protocols

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The following primary antibodies were used: mouse anti-cmyc clone 9E10, mouse anti-acetylated-α-tubulin clone 6-11B-1, mouse anti-HA (Sigma-Aldrich Co. Ltd.), rabbit anti-GFP (‘Living Colors A.v. Peptide Antibody’) and mouse anti-UBE2E1 (BD Biosciences Inc); rabbit-anti-γ-tubulin and mouse anti-β-actin clone AC-15 (Abcam Ltd.); mouse anti-cyclin D1 clone A-12 (Santa Cruz Biotechnology Inc); rabbit anti-phospho-β-catenin and rabbit anti-β-catenin (Cell Signalling Technology Inc); and mouse anti-mono- and polyubiquitinylated conjugates clone FK2 and rabbit anti-20S proteasome α7 subunit (Enzo Life Sciences Inc). Rabbit anti-MKS1 has been described previously (Dawe et al., 2007 (link); Näthke et al., 1996 (link)). Secondary antibodies were AlexaFluor488-, and AlexaFluor568-conjugated goat anti-mouse IgG and goat anti-rabbit IgG (Molecular Probes Inc) and HRP-conjugated goat anti-mouse immunoglobulins and goat anti-rabbit immunoglobulins (Dako Inc).
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