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αcd69 apc

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αCD69-APC is a fluorochrome-conjugated monoclonal antibody that binds to the CD69 antigen. CD69 is a type II membrane protein that is rapidly expressed on the surface of activated lymphocytes, including T cells, B cells, and natural killer cells. The APC fluorochrome emits light in the far-red/near-infrared spectrum, which can be detected using flow cytometry.

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Lab products found in correlation

Macsplex cytokine 12 kit, by Miltenyi Biotec (1 mentions)

2 protocols using αcd69 apc

1

Cytokine and Exhaustion Profiling of Engineered T Cells

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For analysis of the activation and exhaustion status as well as the cytokine release, 5 × 104 genetically modified T cells were incubated alone or together with 1 × 104 target cells in the absence or presence of 25 nM of corresponding TM in 96-well plates in triplicates. After 24 h of co-cultivation, triplicates of cell-free supernatants were harvested and pooled to determine cytokine concentrations with the MACSPlex Cytokine 12 kit (Miltenyi Biotec GmbH) as described previously31 (link),32 (link). In parallel, triplicates of co-cultured T cells were pooled and were stained with αCD69-APC, αPD1-PE, αCD4-PE-Vio770, and αCD8-VioBlue mAbs (Miltenyi Biotec GmbH) and analysed by flow cytometry.
Jureczek J., Bergmann R., Berndt N., Koristka S., Kegler A., Puentes-Cala E., Soto J.A., Arndt C., Bachmann M, & Feldmann A. (2019). An oligo-His-tag of a targeting module does not influence its biodistribution and the retargeting capabilities of UniCAR T cells. Scientific Reports, 9, 10547.
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2

Multiparametric Flow Cytometry Analysis

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αNKP30-PE (Biolegend), αCD56-Fitc (Becton Dickinson), αCD69-APC (Miltenyi) and respective isotype controls from the same companies were used for the analysis of the surface expression.
Marischen L., Englert A., Schmitt A.L., Einsele H, & Loeffler J. (2018). Human NK cells adapt their immune response towards increasing multiplicities of infection of Aspergillus fumigatus. BMC Immunology, 19, 39.
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