Mini protean tgx pre cast gel 7.5

Dulbecco’s modified Eagle’s medium (DMEM) was bought from GIBCO (Thermo Fisher Scientific, Waltham, MA, USA). Fetal bovine serum (FBS) was from Hyclone Laboratories (Logan, UT, USA). Stable l-glutamine, 1% non-essential amino acids, penicillin/streptomycin, and chemiluminescent reagent were from Euroclone (Milan, Italy). Polycarbonate filters, 12 mm diameter, 0.4 µm pore diameter were from Transwell Corning Inc. (Lowell, MA, USA). Phenol red, PBS, bovine serum albumin (BSA), RIPA buffer, and the antibody against β-actin were from Sigma-Aldrich (St. Louis, MO, USA). The antibody against HMGCoAR was bought from Abcam (Cambridge, UK), that against PCSK9 from GeneTex (Irvine, CA, USA), that against phospho-HMGCoAR (Ser872) from Bioss Antibodies (Woburn, MA, USA), and that against LDLR from Pierce (Rockford, IL, USA). The antibodies against SREBP-2, rabbit Ig-HRP, mouse Ig-HRP, phenylmethanesulfonyl fluoride (PMSF), Na-orthovanadate inhibitors were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA); the inhibitor cocktail Complete Midi from Roche (Basel, Switzerland). Mini protean TGX pre-cast gel 7.5% and Mini nitrocellulose Transfer Packs were purchased from BioRad (Hercules, CA, USA). The human proprotein convertase 9 immunoassay (Quantikine ELISA) was bought from R & D System (Minneapolis, MN, USA).

Dulbecco’s modified Eagle’s medium (DMEM), L-glutamine, fetal bovine serum (FBS), phosphate-buffered saline (PBS), penicillin/streptomycin, chemiluminescent reagent and 24- or 96-well plates were purchased from Euroclone (Milan, Italy). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DPPH (1,1-diphenyl-2-picrylhydrazyl), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), TPTZ, Griess reagent, bovine serum albumin (BSA), RIPA buffer, the antibody against β-actin, the fluorometric intracellular ROS kit and MDA assay kit were bought from Sigma-Aldrich (St. Louis, MO, USA). Phenylmethanesulfonylfluoride (PMSF), Na-orthovanadate inhibitors and the antibodies against rabbit Ig-horseradish peroxidase (HRP) and mouse Ig-HRP were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). The iNOS primary antibody came from Cell Signaling Technology (Danvers, MA, USA); the inhibitor cocktail Complete Midi from Roche (Basel, Swiss); mini-protean TGX pre-cast gel 7.5% and mini-nitrocellulose Transfer Packs from Bio-Rad (Hercules, CA, USA). Ultrapure water was produced using a Milli-Q System (Millipore SA, Molsheim, France). Acetonitrile with a HPLC grade was from Panreac (Barcelona, Spain); formic acid was from Merck (Darmstadt, Germany). Standards of quercitrin (>98.5%) and ursolic acid (>98.0%) were from Extrasynthese (Genay, France).