Cells were lysed using boiling lysis buffer (1% SDS, 10 mM Tris.HCl, pH7.4). Protein concentrations were measured using BCA protein reagent (Thermo Scientific) and equal amounts of protein were separated on 10% or gradient polyacrylamide gels and transferred onto PVDF membranes [69 (link), 81 (link)]. The following antibodies were used: rabbit antibodies against phospho-pS6K(T389), phospho-S6(S235/236) and phospho-S6(S240/244), S6K, phospho-4EBP1(T37/46), phospho-AKT(S473) and phospho-AKT(T308), AKT and mouse anti-S6 – from Cell Signaling Technology (Danvers, MA); mouse monoclonal antibodies against cyclin D1 and rabbit anti-actin were from Santa Cruz Biotechnology (Paso Robles, CA) and Sigma-Aldrich (St. Louis, MO), respectively.
Phospho 4ebp1 t37
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-4EBP1(T37) is a laboratory product that detects phosphorylation of the 4E-binding protein 1 (4EBP1) at the threonine 37 residue. This phosphorylation event is a key regulatory mechanism of 4EBP1 function.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein reagent, by Thermo Fisher Scientific (1 mentions)
Phospho akt s473, by Cell Signaling Technology (1 mentions)
Mouse anti s6, by Cell Signaling Technology (1 mentions)
Puromycin dihydrochloride, by Merck Group (1 mentions)
Hexadimethrine bromide, by Merck Group (1 mentions)
Chloroquine diphosphate, by Merck Group (1 mentions)
Ha probe, by Santa Cruz Biotechnology (1 mentions)
Phospho gsk3β, by Cell Signaling Technology (1 mentions)
Phospho ulk1 s757, by Cell Signaling Technology (1 mentions)
Phospho p70s6k t389, by Cell Signaling Technology (1 mentions)
Phospho tuberin tsc2, by Cell Signaling Technology (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Lc3a b, by Cell Signaling Technology (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
P70s6k, by Cell Signaling Technology (1 mentions)
N acetyl l cysteine, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using phospho 4ebp1 t37
1
Cell Lysis and Western Blot Analysis
2
Antibody-based Analysis of Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies to Akt, phospho-Akt(S473), phospho-Akt(T308), phospho-Tuberin/TSC2(T1462), Tuberin/TSC2, GSK-3β, phospho-GSK-3β(S9), mTOR, Raptor, phospho-4E-BP1(T37/46), p70S6K, phospho-p70S6K(T389), LC3A/B, ULK1, phospho-ULK1(S757) and Anti-Rabbit IgG Fab2 Alexa Fluor (R) 488 conjugate were obtained from Cell Signaling Technology (Beverly, MA, USA). β-Actin and HA-probe antibodies were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Piperlongumine was obtained from Indofine Chemical Company (Hillsborough, NJ, USA). Chloroquine diphosphate, Puromycin dihydrochloride, Hexadimethrine bromide and N-Acetyl-L-Cysteine were obtained from Sigma-Aldrich (St Louis, MO, USA). Bafilomycine A1 was obtained from LC Laboratories (Woburn, MA, USA).
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