Antibodies utilized in this study: mouse anti-Tubulin (GTU-88, Sigma), mouse anti-ORF45 (sc-53883, Santa Cruz), mouse anti-K8 (sc-57889, Santa Cruz), mouse anti-K8.1 (sc-65446, Santa Cruz), mouse anti-ORF26 (2F6B8) (NBP1-47357, Novus Biologicals). Rabbit anti-ORF6 was generously provided by Dr. Gary S. Hayward (Johns Hopkins University). HDLMECs were lysed in 2x Laemmli buffer (Bio-Rad) containing 2-mercaptoethanol, and protein concentration was measured by using a Pierce 660nm Protein Assay Kit. Proteins separated on a 10% acrylamide gel were transferred onto a PVDF membrane, which was then blocked with 5% non-fat dry milk in PBS with 0.1% Tween-20 (PBST), and incubated overnight with primary antibody at 4°C. The next day, the PVDF membrane was washed three times with PBST, incubated with HRP-linked secondary antibody for 1–2 hours at room temperature, washed three times in PBST, and then developed using SuperSignal West Femto (Thermo Scientific), and imaged on a Bio-Rad ChemiDoc Imager.
Mouse anti k8
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom
Mouse anti-K8.1 is an antibody that recognizes the K8.1 antigen. It can be used for the detection of the K8.1 protein in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal west femto, by Thermo Fisher Scientific (1 mentions)
Laemmli buffer, by Bio-Rad (1 mentions)
Chemidoc imager, by Bio-Rad (1 mentions)
Rabbit anti flag, by Merck Group (1 mentions)
Rabbit anti gapdh 2118, by Cell Signaling Technology (1 mentions)
Ap307p, by Merck Group (1 mentions)
Mouse anti brdu, by Thermo Fisher Scientific (1 mentions)
F7425, by Merck Group (1 mentions)
Mouse anti β actin ac 15, by Merck Group (1 mentions)
Pa522819, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse anti k8
1
Western Blot Analysis of KSHV Proteins
2
Antibody Panel for KSHV Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies used in this study include rabbit anti-Syncytin-1 (for western blot, PA522819; Invitrogen), rabbit anti-Syncytin-1 (for flow cytometry, PA522819; Invitrogen), mouse anti-bZIP (sc69797; Santa Cruz), mouse anti-K8.1 (sc65446; Santa Cruz), rabbit anti-KSHV RTA (a gift from Yoshihiro Izumiya at University of California Davis), mouse anti-β-actin (AC-15; Sigma), rabbit anti-GAPDH (2118S; Cell Signaling), mouse anti-EA-D (MAB8186; EMD), mouse IgG1 negative isotype (CBL610; EMD Millipore), mouse anti-ZEBRA (a gift from Paul Farrell at Imperial College London, London, United Kingdom), rabbit anti-FLAG (F7425; Millipore), rat anti-EBNA2 (MABE8; Millipore), mouse anti-BrdU (555627; Thermo Fisher Scientific), mouse anti-CD19-APC (MABF197; Sigma-Aldrich), mouse anti-CD20-PE (MABF1635; Sigma-Aldrich), mouse IgG1-APC isotype (550824; BD Pharmingen), rat IgG2a isotype (MABF1077Z; EMD Millipore), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H + L) (AP308P; EMD Millipore), HRP-conjugated goat anti-rabbit IgG (AP307P; EMD Millipore), FITC-conjugated goat anti-mouse IgG (F0257; Sigma), and APC-conjugated goat anti-rabbit IgG. All antibodies were used at concentrations and conditions recommended by manufacturers.
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