Dako envision system hrp labelled polymer kit

Immunohistochemistry was performed on FFPE tissue slides following a 45-minute antigen retrieval step (pH 6). To stain human CD147 (hCD147), the Dako EnVision + System- HRP Labelled Polymer kit (Agilent) was used according to manufacturer’s instructions against slides incubated with primary mouse anti-human CD147 antibody (Biolegend, HIM6; 1:500). To stain mouse CD147 (mCD147), polyclonal donkey anti-goat IgG secondary (Jackson ImmunoResearch, 705–035-003 [HRP]; 1:500) was used against primary goat anti-mouse CD147 antibody (R&D Systems, BAF772; 1:100). Slides were then incubated with 3,3′-Diaminobenzidine (DAB) for 4 minutes before washing and counterstained with Gill No. 2 hematoxylin. Slides were then dehydrated and mounted.

Immunohistochemistry (IHC) was performed on FFPE tissue slides following a 45-minute antigen retrieval step (pH 6.0). To stain human CD147 (hCD147) and SARS-CoV-2 Spike protein RBD, the Dako EnVision + System- HRP Labelled Polymer kit (Agilent) was used according to manufacturer’s instructions against slides incubated with primary mouse anti-human CD147 antibody (Biolegend, HIM6; 1:500) or primary rabbit anti-RBD antibody (Sino Biological; 40592-T62; 1:800), respectively. To stain mouse CD147 (mCD147), polyclonal donkey anti-goat IgG secondary (Jackson ImmunoResearch; 705-035-003 [HRP]; 1:500) was used against primary goat anti-mouse CD147 antibody (R&D Systems; BAF772; 1:100). Slides were then incubated with 3,3’-Diaminobenzidine (DAB) for 4 minutes before washing and counterstained with Gill No. 2 hematoxylin. Slides were then dehydrated and mounted for imaging.