Anti atf3

Briefly, the cells were lysed in radioimmunoprecipitation (RIPA) buffer (150 mM NaCl, 1.0% (v/v) NP-40, 0.5% (w/v) sodium deoxycholate, 0.1% (v/v) sodium dodecyl sulfate (SDS), 50 mM Tris-HCl (pH 8.0), and Roche Complete Mini EDTA-free) for 30 min. They were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for WB. The following primary antibodies were used: anti-ATF3 (1:1,000; #18665; Cell Signaling Technology (CST), Danvers, MA, USA), anti-PERK (1:1,000; #5683; CST), anti-ATF6 (1:1,000; #65880; CST), and anti-IRE1α (1:1,000; #3294; CST).

Sulforhodamine B, tunicamycin, and GSH-EE were purchased from Sigma‒Aldrich (St. Louis, MO, USA). A genomic DNA kit was obtained from Promega (Madison, WI, USA). The anti-β-Tubulin (#2128), anti-ATF3 (#18665), anti-GADD45A (#4632), anti-ATF6 (#65880), anti-CHOP (#2895), anti-GRP78 (#3177), anti-XBP1 (#40435), anti-IRE1α (#3294), anti-DR5 (#8074), anti-Bax (#2772), anti-ATF4 (#11815), anti-Puma (#4976), anti-PERK (#3192), anti-p-eIF2α (#9721), anti-eIF2α (#9722), anti-p-JNK (#9251), anti-JNK (#9252), anti-cytochrome c (#4272), anti-COXIV (#4850), anti-Calnexin (#2679), anti-PARP1 (#9542), anti-Poly/Mono-ADP Ribose (#83732), anti-Caspase 3 (#9662), anti-Caspase 8 (#9746), and anti-Caspase 9 (#9502) antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). The anti-PARP16 antibody (ab154510) was obtained from Abcam (Cambridge, UK), the anti-Sestrin2 antibody (10795-1-AP) was obtained from Proteintech (Rosemont, IL, USA), the anti-CYB5R3 antibody (BS-12162R) was obtained from Bioss Antibodies Inc. (Woburn, MA, USA), the anti-GAPDH antibody (LF-P-A0212) was obtained from AbFrontier (Seoul, Korea), the anti-ARTC1 (SAB1300652) and anti-Flag (F1804) antibodies were obtained from Sigma‒Aldrich, and the mouse anti-FITC (sc-2010) and rabbit anti-rhodamine (sc-2492) antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA).