Purified anti mouse cd28 mab

Purified anti-mouse CD28 mAb, anti-mouse CD3 mAb, FITC-conjugated anti-mouse CD4 and PE-conjugated anti-mouse CD69 were purchased from BioLegend (San Diego, CA, USA). The Seahorse XF Glycolysis Stress Test Kit and Mitochondrial Stress Test Kit were provided by Agilent Technologies (Palo Alto, Calif.). Roswell Park Memorial Institute (RPMI) 1640, fetal bovine serum (FBS) and penicillin/streptomycin were ordered from Gibco (South Logan, UT, USA). DAPI (4′,6-diamidino-2-phenylindole) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) were purchased from Solarbio (Beijing, China). Enhanced ATP Assay Kit, Mitochondrial Membrane Potential Detection Kit and Reactive Oxygen Detection Kit were supplied by Beyotime Biotechnology (Shanghai, China).

Recombinant mouse IL-6, purified anti-mouse CD28 mAb, anti-mouse CD3 mAb, FITC conjugated anti-mouse CD4, APC-conjugated anti-mouse IFN-γ, and PE-conjugated anti-mouse IL-17, anti-mouse IL-4 mAb, anti-mouse IFN-γ mAb and IL-17 ELISA kits were bought from BioLegend (San Diego, CA, USA), Recombinant human TGFβ1.2 was obtained from eBioscience (San Diego, CA, USA). L-lactate detection kit was obtained from Eton bioscience (San Diego, CA, USA). The LADH inhibitor GNE-140 was purchased from MCE (Minneapolis, MN). Pertussis toxin was obtained from List Biological Lab (Campbell, CA, USA). The glycolysis stress test kit and mitochondria stress test kit were obtained from Agilent Technologies (Palo Alto, Calif.). The myelin oligodendrocyte glycoprotein (MOG) 35-55 was purchased from GL Biochem Corporation (Shanghai, China). Iscove’s Modified Dulbecco Medium (IMDM), Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were ordered from Gibco (South Logan, UT, USA). Non-viable, desiccated Mycobacterium tuberculosis H37 RA and incomplete Freund adjuvant (IFA) was bought from BD Difco (Detroit, MI, USA); L-sodium lactate, Phorbol 12-myristate 13-acetate and Ionomycin were bought from Sigma Aldrich Company (St. Louis, MO).