Mycoplasma PCR was performed using the PCR Mycoplasma Test Kit I/C (PK-CA91-1024, PromoCell, Heidelberg, Germany) according to the manufacturer's instructions. The kit includes a Olympus) equipped with a Color Mosaik 18.2 camera (Visitron Systems) and the SPOT Advanced software (version 4.6.3.8) . Every time, overview (40x magnification: Olympus UPlanFLN, 4x/0.3 PhP) and close-up pictures (200x magnification: Olympus LCAchN, 20x/0.40 PhP) were taken and archived.
Lcachn
Manufactured by Olympus
Sourced in Germany
The LCAchN is a laboratory equipment product from Olympus. It is designed to perform core functions related to the analysis and processing of samples in a laboratory setting. The detailed specifications and intended uses of this product are not available for an unbiased and factual description within the requested constraints.
Automatically generated - may contain errors
Lab products found in correlation
Uplanfln, by Olympus (4 mentions)
Color mosaik 18.2 camera, by Visitron (2 mentions)
Pcr mycoplasma test kit 1 c, by PromoCell (2 mentions)
Cellsens standard, by Olympus (1 mentions)
Uplanfi, by Olympus (1 mentions)
Bx43 microscope, by Olympus (1 mentions)
6 well plate, by Sarstedt (1 mentions)
Cellsens software, by Olympus (1 mentions)
Flourobrite medium, by Thermo Fisher Scientific (1 mentions)
4 protocols using lcachn
1
Mycoplasma Detection by PCR Imaging
2
TUNEL Staining Quantification Protocol
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TUNEL staining was observed with 20× (UPlanFI; Olympus) and 40× (UPlanFLN; Olympus) objectives, and images were acquired using a fluorescence microscope (model BX41; Olympus) and camera (model XM10; Olympus) at room temperature with a DAPI or FITC filter. 3–10 immunofluorescent images per mouse were randomly photographed, and at least 1,000 DAPI-stained cells per mouse were counted. Quantitative results were generated from the counted numbers in 3–6 mice from independent experiments. Bright-field images were acquired with a 20× (LCAchN; Olympus) objective using an inverted microscope (model CKS41; Olympus) at room temperature. Image analysis was performed using cellSens Standard (Olympus) image analysis software.
3
Fluorescence Microscopy of pHEMA Samples
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Cell imaging was carried out using fluorescence microscopy, phase contrast microscopy, and bright field microscopy. A BX 43 microscope using the IC capture software (Olympus, Hamburg, Germany) with reflection and fluorescence microscopy using different objectives (10× UPLanFLN, 20× LcACH N; Olympus, Hamburg, Germany) was used to image the pHEMA samples. For the controls, i.e., cells on 6-well plates (Sarstedt, Germany), an inverted microscope (IX81) and CellSens software (Olympus, Hamburg, Germany); objectives: 10× UPLanFLN, 20× LcACH N (Olympus, Hamburg, Germany) was used. For each position, images in reflection and fluorescence mode were recorded. The imaging was conducted in FlouroBrite medium (Gibco, Germany) to limit background fluorescence.
4
Mycoplasma Detection by PCR Imaging
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Mycoplasma PCR was performed using the PCR Mycoplasma Test Kit I/C (PK-CA91-1024, PromoCell, Heidelberg, Germany) according to the manufacturer's instructions. The kit includes a Olympus) equipped with a Color Mosaik 18.2 camera (Visitron Systems) and the SPOT Advanced software (version 4.6.3.8) . Every time, overview (40x magnification: Olympus UPlanFLN, 4x/0.3 PhP) and close-up pictures (200x magnification: Olympus LCAchN, 20x/0.40 PhP) were taken and archived.
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