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Di multimode 5

Manufactured by Bruker

The DI MultiMode V is a scanning probe microscope (SPM) system developed by Bruker. It is designed for high-resolution imaging and analysis of surfaces at the nanoscale level. The core function of the DI MultiMode V is to provide users with the ability to capture detailed topographical and physical property information of a wide range of samples.

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2 protocols using di multimode 5

1

AFM Imaging of Biomolecules on Mica

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AFM images were obtained using a Veeco DI MultiMode V instrument in air using scan assist mode with SCANASYST-AIR tips (Bruker). Two μL of sample and 48 μL of 1× TAE with 12.5 mM Mg2+ buffer were pipetted onto freshly cleaved mica and allowed to adhere to the surface for 2 min. Samples were washed with 50 μL of water to remove excess salts and immediately dried with a stream of air.
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2

Structural Characterization of Samples

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The scanning electron microscopy (SEM) images of the samples were acquired on a field-emission scanning electron microscope (Zeiss, Sigma, Germany). Atomic force microscopy (AFM; DI Multimode V, Bruker Co.) and smallangle X-ray scattering (SAXS; ee-MC2 X-ray diffractometer) were employed for detection of the morphology structure of
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