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2 protocols using goat anti snap25

1

Comprehensive Western Blot Analysis of Neurological Markers

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The following primary antibodies were used in the western blot analysis.
Rabbit-anti-synaptophysin, anti-caspase-3, anti-cleaved casapse-3,
anti-phospho-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid
receptors) AMPAR1s (p-GluR1) Ser845), anti-PSD-95, anti-p-PI3K (Y458/Y199),
total anti-PI3K, anti-p-Akt (Ser473), anti-total Akt, anti-caspase-9, anti-total
tau, and anti-β-actin from Cell Signaling Technology, Beverly, MA,USA.
The mouse-anti-Aβ (D-11), rabbit-anti-BACE-1, goat-anti-SNAP25,
goat-anti-pGSK3 β(Ser9), rabbit-anti-total GSK3β,
mouse-anti-total GluR1, rabbit-anti-p-Tau (Ser 413), mouse-anti-p53,
mouse-anti-poly (ADP-ribose) polymerase-1(PARP-1) from Santa Cruz,
Biotechnology, CA,USA.
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2

Western Blot and ChIP Antibody Characterization

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The antibodies for Western blot analysis used were: mouse anti-α-tubulin (1:10,000; Sigma, clone B512), rat anti-α-synuclein (1:100; clone 15G7), mouse anti-α-synuclein (1:1000; BD, Synuclein-1), rabbit anti-GFP (1:1000; Santa Cruz), rabbit anti-H3 (1:16000; abcam), rabbit anti-H3K4me3 (1:16000; Abcam), rabbit anti-H3K9me1 (1:16000; Abcam), rabbit anti-H3K9me2 (1:4000; CST), rabbit anti-H3K9me3 (1:16000; Abcam), rabbit anti-H3K27me3 (1:8000; Millipore), rabbit anti-H3K9ac (1:8000; Millipore), rabbit anti-H3K14ac (1:8000; EpiGentek), rabbit anti-H4 (1:16000; Abcam), rabbit anti-H4K12Ac (1:16000; Abcam), rabbit anti-HP1α (1:1000; CST), rabbit anti-EHMT2 (1:2000; abcam), rabbit anti-p38 (1:1000; CST), goat anti-REST (1:250; Santa Cruz) and goat anti-SNAP25 (1:1000; Santa Cruz). The HRP-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories (1:10,000). The antibodies for chromatin immunoprecipitation (ChIP) used were: rabbit anti-EHMT2 (10 μg; Millipore), mouse anti-H3K9me2 (4 μg; Abcam), and rabbit anti-REST (10 μg; Millipore).
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