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Milli q merck

Manufactured by Merck Group
Sourced in Germany, Chile

Milli-Q Merck is a water purification system that produces ultrapure water. It removes impurities from water through a multi-stage purification process, including filtration, ion exchange, and UV treatment. The system is designed to provide high-quality water for use in various laboratory applications.

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4 protocols using milli q merck

1

Pumpkin Flower Foam-Mat Antioxidant Assays

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All the solutions employed were prepared with deionized water (Milli-Q Merck, Millipore Darmstadt, Hesse, Germany) with a resistivity of 18.2 MΩ cm or greater. All chemicals used were analytical grade and used without further purification. Reagents employed for the pumpkin flower foam-mat elaboration; maltodextrin, hydroxyethyl cellulose, and egg albumin (EA) were purchased from Food Technologies Trading (Estado de Mexico, Mexico), Tween-80 was purchased from J.T. Baker (Phillipsburg, NJ, USA).
Reagents employed for the antioxidant activity assays; 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), potassium persulfate, 2,4,6-tris-2-piridil-s-triazine (TPTZ), hydrochloric acid, acetic acid, Folin–Ciocalteu reagent, gallic acid, thiobarbituric acid (TBA), 1,1,3,3-tetraethoxypropane (TEP), and methanol (MeOH) were purchased from Sigma Aldrich (St. Louis, MO, USA). ABTS was purchased from Roche Diagnostics (Indianapolis, IN, USA). Trichloroacetic acid (TCA) was purchased from Meyer (Estado de Mexico, Mexico). Iron (II) sulfate, sodium carbonate, and sodium acetate were obtained from J.T. Baker (Phillipsburg, NJ, USA). Ferric (III) chloride was purchased from Merck (Darmstadt, Hesse, Germany).
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2

Sensitive Leucomalachite Green Quantification

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All the solutions were prepared using deionized water (Milli-Q Merck, Millipore Darmstadt, Hesse, Germany) with a resistivity of 18.2 MΩ cm or greater. All chemicals used were analytical grade and used without further purification. Leucomalachite green (LMG), malachite green (MG), NaHCO3, glacial acetic acid, NaCl and ACN were purchased from Sigma Aldrich (St. Louis Missouri, USA). Copper phthalocyanine dye was obtained from Santa Cruz chemicals (Huissen, Gelderland, Netherlands), ammonium acetate and Tween-80 were purchased from J.T. Baker (Phillipsburg, New Jersey, USA).
Working standard solutions (5.0 mM) were prepared by dissolving the leucomalachite green in a buffer solution. The buffer solution consisted of 50 mM ammonium acetate, ACN (40% v/v), Tween-80 (1% v/v) as surfactant and the pH value was adjusted with acetic acid to pH = 4.0 ± 0.02. The carrier solution used for μFIA experiments consisted of 50 mM ammonium acetate, MeOH (20% v/v), Tween-80 (3% v/v) and the pH value was adjusted to 4.00 ± 0.02 with acetic acid.
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3

UHPLC-MS Analysis of Biomolecules

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UHPLC-MS solvents were purchased from Merck (Santiago, Chile). Ultrapure water was obtained from a Millipore water purification system (Milli-Q Merck Millipore, Chile). Some standards were purchased either from Sigma Aldrich (Saint Louis, Mo, USA), or Extrasynthèse (Genay, France).
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4

Thin Layer Chromatography and Column Chromatography

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Thin layer chromatography was developed in n-heptane/EtOAc mixtures; spots were revealed by spraying plates with v/v) , and heating at 120°C. Silica gel (Merck 0.063-0.200 mm) was used for column chromatography (CC). Technical solvents used in chromatography processes were previously distilled and dried according to standard procedures. Ethyl lactate (EL) and n-heptane were purchased from Sigma Aldrich (Saint Louis, Mo, USA). UHPLC-MS solvents were purchased from Merck (Santiago, Chile). Ultrapure water was obtained from a Millipore water purification system (Milli-Q Merck Millipore, Chile).
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