Reagents employed for the antioxidant activity assays; 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), potassium persulfate, 2,4,6-tris-2-piridil-s-triazine (TPTZ), hydrochloric acid, acetic acid, Folin–Ciocalteu reagent, gallic acid, thiobarbituric acid (TBA), 1,1,3,3-tetraethoxypropane (TEP), and methanol (MeOH) were purchased from Sigma Aldrich (St. Louis, MO, USA). ABTS was purchased from Roche Diagnostics (Indianapolis, IN, USA). Trichloroacetic acid (TCA) was purchased from Meyer (Estado de Mexico, Mexico). Iron (II) sulfate, sodium carbonate, and sodium acetate were obtained from J.T. Baker (Phillipsburg, NJ, USA). Ferric (III) chloride was purchased from Merck (Darmstadt, Hesse, Germany).
Milli q merck
Milli-Q Merck is a water purification system that produces ultrapure water. It removes impurities from water through a multi-stage purification process, including filtration, ion exchange, and UV treatment. The system is designed to provide high-quality water for use in various laboratory applications.
Lab products found in correlation
4 protocols using milli q merck
Pumpkin Flower Foam-Mat Antioxidant Assays
Reagents employed for the antioxidant activity assays; 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), potassium persulfate, 2,4,6-tris-2-piridil-s-triazine (TPTZ), hydrochloric acid, acetic acid, Folin–Ciocalteu reagent, gallic acid, thiobarbituric acid (TBA), 1,1,3,3-tetraethoxypropane (TEP), and methanol (MeOH) were purchased from Sigma Aldrich (St. Louis, MO, USA). ABTS was purchased from Roche Diagnostics (Indianapolis, IN, USA). Trichloroacetic acid (TCA) was purchased from Meyer (Estado de Mexico, Mexico). Iron (II) sulfate, sodium carbonate, and sodium acetate were obtained from J.T. Baker (Phillipsburg, NJ, USA). Ferric (III) chloride was purchased from Merck (Darmstadt, Hesse, Germany).
Sensitive Leucomalachite Green Quantification
Working standard solutions (5.0 mM) were prepared by dissolving the leucomalachite green in a buffer solution. The buffer solution consisted of 50 mM ammonium acetate, ACN (40% v/v), Tween-80 (1% v/v) as surfactant and the pH value was adjusted with acetic acid to pH = 4.0 ± 0.02. The carrier solution used for μFIA experiments consisted of 50 mM ammonium acetate, MeOH (20% v/v), Tween-80 (3% v/v) and the pH value was adjusted to 4.00 ± 0.02 with acetic acid.
UHPLC-MS Analysis of Biomolecules
Thin Layer Chromatography and Column Chromatography
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