SchB was purchased from aladdin (Shanghai, China). MEM-α was purchased from Gibco (Beijing, China). Fetal Bovine Serum was from Gemini bio-products (USA). Trypsin-EDTA solution and DMSO were from Solarbio (Beijing, China). Penicillin–Streptomycin solution was from HyClone (USA). TrIzol Reagent, RevertAid First Strand cDNA Systhesis kit and PowerUp SYBR Green Master Mix were purchased from Thermo Fisher Scientific (USA). RIPA buffer and PMSF were purchased from Solarbio (Beijing, China). BMP2 (Cat#18933-1), SMAD1 (Cat#10429-1-AP), SMAD4 (Cat#10231-1-AP), SMAD5 (Cat#12167-1-AP), SMAD9 (Cat#16397-1-AP) rabbit polyclonal antibody, HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) and HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) Antibody were purchased from proteintech. RUNX2 (D1L7F) Rabbit mAb was purchased from cell signaling technology (Cat#12556). SP7 Polyclonal antibody was purchased from Invitrogen (Cat#PA5-40509). Chemiluminescent HRP substrate was purchased from Millipore.
Runx2 d1l7f rabbit mab
Manufactured by Cell Signaling Technology
Sourced in United States
RUNX2 (D1L7F) Rabbit mAb is an antibody product developed by Cell Signaling Technology. It is designed to detect endogenous levels of RUNX2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Trypsin edta solution, by Solarbio (1 mentions)
Chemiluminescent hrp substrate, by Merck Group (1 mentions)
Smad4, by Proteintech (1 mentions)
Smad5, by Proteintech (1 mentions)
Hrp conjugated affinipure goat anti rabbit igg h l, by Proteintech (1 mentions)
Revertaid first strand cdna systhesis kit, by Thermo Fisher Scientific (1 mentions)
Smad1, by Proteintech (1 mentions)
α mem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions)
Ripa buffer, by Solarbio (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Hrp conjugated affinipure goat anti mouse igg h l, by Proteintech (1 mentions)
Penicillin g streptomycin sulfate, by Thermo Fisher Scientific (1 mentions)
Cell light edu apollo 488 in vitro imaging kit, by RiboBio (1 mentions)
6 well culture plates, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
2 protocols using runx2 d1l7f rabbit mab
1
Bone Differentiation Pathway Evaluation
2
Osteogenic Differentiation of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fetal bovine serum (FBS), α-minimum essential medium (αMEM), trypsin-EDTA, phosphate-buffered saline (PBS) and penicillin G-streptomycin sulfate were purchased from Gibco® Life Technologies. U0126 (ERK1/2 inhibitor), 2,7-dichlorodihydrofluoresceindiacetate (DCFH-DA), β-glycerophosphate, ascorbic acid, and dexamethasone were purchased from Sigma–Aldrich (USA). Transwells (6-Well Millicell Hanging Cell Culture Inserts, 0.4 μm, PET) and 6-well culture plates were purchased from Millipore® (USA). The rabbit anti-phospho-Runx2 (Ser451) was purchased from BIOSS Bioscience (China).The Cell-Light™ EdU Apollo®488 In Vitro Imaging Kit was purchased from RiboBio (China).The goat anti-rabbit IgG, phospho-p44/42 MAPK rabbit mAb(p-ERK1/2), p44/42 MAPK (ERK1/2) rabbit mAb, and Runx2 (D1L7F) rabbit mAb were purchased from Cell Signaling Technology (USA).The protein assay kit, RIPA buffer, and DAPI were purchased from Beyotime (China). The alkaline phosphatase (ALP) assay kit and bicinchoninic acid (BCA) assay kit were purchased from the Jiancheng Corp (China).Other reagents used were of the highest commercial grade available.
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