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2 protocols using n lauroyl d erythro sphingosine

1

Synthesis and Characterization of Ceramides

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N-lauroyl-D-erythro-sphingosine (C12 : 0 CER), N-palmitoyl-D-erythro-sphingosine (C16 : 0 CER), and N-lignoceroyl-D-erythro-sphingosine (C24 : 0 CER) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). CER from bovine brain was obtained from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of reagent, HPLC, or LC-MS grade.
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2

Lipidomics Analysis of Sphingolipids

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Propan-2-one, methanol, 1-butanol, LC-MS grade water, primuline yellow dye, ammonium dihydrogen phosphate, 3,5-Di-tert-4-butylhydroxytoluene (BHT) and ammonium formate were from Sigma-Aldrich (Saint Louis, MO, USA). Ethanol and high performance liquid chromatography (HPLC)-analytical grade chloroform (CHCl3) were respectively from J.T. Baker (Center Valley, PA, USA) and Carlo Erba (Cornaredo, MI, Italy). N-lignoceroyl-D-erythro-sphingosine (Cer C24:0) 1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine (DPPC), Cardiolipin (CL), 1,2-Dipalmitoyl-sn-Glycero-3-Phosphoethanolamine (DPPE), D-glucosyl-ß-1,1′-N-stearoyl-D-erythro-sphingosine-d5 (HexCer), N-lauroyl-D-erythro-sphingosine, N-lauroyl-D-erythro-sphinganine, N-lauroyl-D-erythro-sphingosylphosphorylcholine N-lauroyl-D-erythro-sphinganylphosphorylcholine, D-glucosyl-ß−1,1′-N-lauroyl-D-erythro-sphingosine, D-lactosyl-ß-1,1′ N-lauroyl-D-erythro-sphingosine and C17 d-erythro-dihydrosphingosine-1-phosphate lipids standards were from Avanti Polar Lipids (Alabaster, Alabama, USA). SL standard mixture, containing SM and Sulfatides (SLF) was from Matreya LLC (Pleasant Gap, PA, USA).
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