Sgc 7901

The GES‐1 cell line was provided by the Shanghai Institute of Cell Biology, Chinese Academy of Sciences. The AGS, SGC‐7901, and SK‐GT5 cell lines were purchased from ATCC. SGC‐7901 and SK‐GT5 cells were cultivated in Dulbecco's modified Eagle's medium (DMEM) and an environment of 5% CO₂ at 37°C, and GES‐1 cells were cultivated in Roswell Park Memorial Institute 1640 (RPMI 1640) medium supplemented with 10% fetal bovine serum (Life Technologies/Gibco), 100 U/mL penicillin, and 100 µg/mL streptomycin (Life Technologies/Gibco). The plasmids of αSR and CagA‐wild type were purchased from Dr Masanori Hatakeyama of Japan's Tokyo University. The pEGFP‐N1 plasmid was purchased from Clontech, the plasmids TET1‐wt and pX459 were obtained from Addgene, and pX459‐TET1‐sgRNA was constructed in the laboratory. The liposome transfection reagent Lipofectamine 3000 was purchased from Invitrogen in the United States. Antibodies against HA were purchased from OriGene, antibodies against CagA were purchased from Santa Cruz, antibodies against 5‐hmC were purchased from Active Motif, and antibodies against KLF4 and TET1 were purchased from Santa Cruz.

Human gastric cancer cell lines including AGS, BGC-823, SGC-7901, and MGC-803, and normal human gastric mucosa epithelial cell line GES-1 were purchased from ATCC, USA. Cells were cultured in DMEM (Thermo Fisher, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher) in a 37°C humidified atmosphere of 5% CO2.

Gastric cancer cell lines (SGC-7901, MKN-45, AGS, MKN-28, and MGC-803) and human normal gastric mucosal epithelial cells (NGEC) were purchased from ATCC (American Type Culture Collection, ATCC). NGEC, AGS cells were cultured in 90% DMEM (Biological Industries, Cat. No. 06-1055-57-1A), 10% FBS (Biological Industries, Cat. No. 04-007-1A), and 1% penicillin/streptomycin. SGC-7901, MKN-45, MKN-28, and MGC-803 cells were cultured in 90% RPMI (Biological Industries, Cat. No. 01-101-1A), 10% FBS, and 1% penicillin/streptomycin. Although MGC-803 cell reports show cross-contamination with HeLa, there are still many research groups using MGC-803 as a model for studying gastric cancer cells (Zhai et al., 2019 (link); Li et al., 2020 (link); Peng et al., 2020 (link)).