Human embryonic kidney (HEK) 293T cells (ATCC # CRL-3216) were grown in DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Jurkat T-lymphocytes cells (ATCC # TIB-152) were grown in RPMI 1640 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated FBS. MCF-7 human breast cancer cells (ATCC # HTB-22) were grown in RPMI 1640 supplemented with 10% heat-inactivated FBS. MCF10A normal human mammary epithelial cells (ATCC # CRL-10317) were grown in DMEM/F12 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated FBS and EGF (0.02 μg/ml), hydrocortisone (0.5 μg/ml), cholera toxin (0.1 μg/ml), insulin (10 μg/ml) (purchased all from Sigma-Aldrich, Milan, Italy). All cultures were grown in a humidified incubator maintained at 37°C with 5% CO2.
Mcf 7 human breast cancer
Manufactured by American Type Culture Collection
Sourced in United States
MCF-7 is a human breast adenocarcinoma cell line derived from the pleural effusion of a 69-year-old Caucasian woman. It is one of the most widely used breast cancer cell lines for in vitro studies.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Cell culture reagents, by Corning (1 mentions)
Sk ov 3 human ovarian cancer, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Thp 1 cell line, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Merck Group (1 mentions)
Nunc microwell plate, by Thermo Fisher Scientific (1 mentions)
Hepg2 human hepatoma, by American Type Culture Collection (1 mentions)
6 protocols using mcf 7 human breast cancer
1
Cell Culture Protocols for Cancer Research
2
Cell Culture Protocol for Cancer and Fibroblast Lines
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SW480 human colon cancer, MCF-7 human breast cancer, SKOV3 human ovarian cancer, HTB-35 human squamous cervical cancer cells and untransformed human HSF2617 and HFF fibroblasts were obtained from the ATCC (Manassas, VA, USA). All cell lines were cultured in DMEM at 37 °C and 5% CO2 pressure. Medium was supplemented with 10% fetal bovine serum, 4 mM glutamine, 1 mM pyruvate, 10 and 25 mM glucose, and 1% (v/v) penicillin and streptomycin in bicarbonate buffer at pH 7.2. Cell culture reagents were obtained from Corning through ThermoFisher (Fairlawn, NJ, USA).
3
Cell Culture Protocol for Cancer and Immune Cells
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MCF-7 human breast
cancer and human monocyte THP-1 cell lines were
obtained from the American Type Culture Collection (ATCC). Human dermal
FB (HDFa) cell line was purchased from ThermoFisher scientific. MCF-7
cells were cultured in Dulbecco’s modified Eagle’s medium
(DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum
(FBS) (Gibco), 1%l -glutamine, 2.7% sodium bicarbonate, 1%
HEPES buffer, and 1% penicillin/streptomycin solution (GPS, Sigma).
FBS were cultured in Human Fibroblast Expansion medium (ThermoFisher
scientific). THP-1 cells were cultured in the Roswell Park Memorial
Institute (RPMI) culture media. Cells were grown at 37 °C in
an atmosphere containing 5% CO2 and 95% humidity. Cell
lines were tested routinely for mycoplasma contamination.
cancer and human monocyte THP-1 cell lines were
obtained from the American Type Culture Collection (ATCC). Human dermal
FB (HDFa) cell line was purchased from ThermoFisher scientific. MCF-7
cells were cultured in Dulbecco’s modified Eagle’s medium
(DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum
(FBS) (Gibco), 1%
HEPES buffer, and 1% penicillin/streptomycin solution (GPS, Sigma).
FBS were cultured in Human Fibroblast Expansion medium (ThermoFisher
scientific). THP-1 cells were cultured in the Roswell Park Memorial
Institute (RPMI) culture media. Cells were grown at 37 °C in
an atmosphere containing 5% CO2 and 95% humidity. Cell
lines were tested routinely for mycoplasma contamination.
4
Human Breast Cancer Cell Culture
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MDA-MB-231 and MCF-7 human breast cancer cells were purchased from the American Type Culture Collection (Manassas, USA). All cells were maintained in Dulbecco's Modified Eagle Medium (DMEM; Life Technologies, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and penicillin/streptomycin (50 U/mL), at 37°C and 5% CO2. Cell lines were tested and confirmed to be free of mycoplasma contamination.
5
Culturing MCF-7 and HCT 116 Cancer Cells
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Cancerous cells MCF-7 human breast cancer and HCT 116 human colon cancer cell lines were obtained from American Type Culture Collection (USA). The cells were cultured and maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco Co. USA) supplemented by 10% fetal bovine serum (FBS) (Sigma, St. Louis, MO, USA) and 1% antibiotic (streptomycin 200 μg/mL and penicillin 100 units/mL) (Gibco Co. USA). Cells were incubated at 37 °C in 5% CO2 environment and then detached and harvested at confluence 80–90% by trypsinization with trypsin-EDTA (Sigma, St. Louis, MO, USA).
6
Anti-proliferative Assay for Cancer Cell Lines
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Two cancer cell lines, HepG2 human hepatoma and MCF-7 human breast cancer cells from the American type Culture Collection (Rockville, MD, USA), were cultured in a 75-cm 2 sterile flat-bottomed bottle containing DMEM supplemented with 10% FBS and 10% of antibiotic-antimycotic in a humidified atmosphere of 5% CO 2 at 37°C until confluent. An anti-proliferative activity assay was performed using the method previously described (27) with some modifications. Cells (5 × 10 5 cells/well) were seeded in 96-well plates (Thermo Scientific™ Nunc™ MicroWell™ Plates with Nunclon™ Delta Surface, Nunc™, Nalge Nunc, Denmark). Various concentrations of the tested sample were added and the plates were incubated at 37°C for 20 h. DMSO was used as a vehicle control. Afterwards, the medium was drained and 100 μL of (5 mg/mL) MTT was added to each well. Plates were further incubated at the same temperature for 4 h. Finally, the cells were dissolved by adding DMSO before measuring the absorbance of the resulting purple solution at 570 nm with a microplate reader (Tecan Sunrise, Software Magellan V.5.00, Switzerland). Absorbance at 630 nm served as a reference. All treatments were done in quadruplicate.
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