All human PDAC cell lines were from the American Type Culture Collection (ATCC). PANC-1 cells and MIA PaCa-2 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM L-glutamine, and 1 mM sodium pyruvate. BxPC-3 cells and AsPC-1 cells were maintained in RPMI 1640 medium supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. HPAF-II cells were maintained in Eagle’s Minimum Essential medium supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. To facilitate bioluminescence imaging (BLI) of tumor growth, PANC-1 cells were permanently transfected with a luciferase-lentiviral vector in the UCLA vector core facility37 (link). Briefly, 1 × 106 PANC-1 cells immersed in 1 mL complete DMEM were transduced with luciferase-lentiviral vector in 6-well tissue culture plates. The viral containing media was removed after 16 h, and the cultures were replenished with fresh DMEM media. Cells were allowed to proliferate to a population size of 5 × 106. Limiting dilution was used to select individual cell that express the highest luciferase. The highest luciferase expressing clone (refers as PANC-1-luc) out of 10 single clones was used for further experiments.
Human pdac cell lines
Manufactured by American Type Culture Collection
Sourced in United States
The Human PDAC cell lines are a collection of cell lines derived from pancreatic ductal adenocarcinoma (PDAC) tissue samples. These cell lines are designed for research purposes and can be utilized in a variety of experimental settings to study PDAC biology and disease pathways.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using human pdac cell lines
1
Establishing Luciferase-Expressing PANC-1 Cells
2
Establishment and Maintenance of PDAC Cell Lines
3
Pancreatic Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We purchased human PDAC cell lines from American Type Culture Collection (ATCC), Rockville, Maryland. Cell lines were authenticated by short tandem repeat (STR) analysis at ATCC, per request, and were mycoplasma-free. CFPAC-1 and Capan-1 cell lines were grown in IMDM with 10% FBS, and 1% penicillin–streptomycin. Capan-2 cell line was grown in McCoy’s 5A (Modified) medium with 10% FBS, and 1% penicillin–streptomycin. The remaining PDAC cell lines were cultured in RPMI 1640 medium with GlutaMax™, 10% FBS, and 1% penicillin–streptomycin in a humidified incubator containing 5% CO2 at 37 °C. All reagents for cell culture were purchased from Thermo Fisher Scientific (Waltham, MA).
4
Authenticating and Culturing PDAC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human PDAC cell lines were purchased from the American Type Culture Collection (ATCC) and were authenticated by DNA fingerprinting at the University of Texas MD Anderson Characterized Cell Line Core. Cells were cultured as previously described14. Antibodies were purchased from Cell Signaling Technologies (Danvers, MA), Invitrogen (Carlsbad, CA), Sigma-Aldrich (St. Louis, MO), Santa Cruz Biotechnology (Dallas, TX), and Abcam (Cambridge, UK). Details of the antibodies are shown in S1 Table . The STAT-3 inhibitor was supplied by David Tweardy and was previously characterized in Bharadwaj et al. [21 (link)].
5
3D Mini-Organotypic Model of PDAC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human PDAC cell lines were obtained from the American Type Culture Collection (ATCC, Rockville, USA). PS1 cell line (immortalized non-tumoural PSC) was developed and characterized previously in our laboratory39 (link). The mini-organotypic model37 (link), was used to examine the effect of genetic manipulation or ATRA treatment on PDAC cells (MiaPaCa2 and CaPan1) and PSC (PS1) co-cultured in 3D, physio-mimetic environment. Mini-organotypic cultures were incubated for 7 days before being harvested, fixed in 10% neutral buffered formalin (BAF‐0010‐03 A; CellPath Ltd), submerged in 70% ethanol, embedded in paraffin and cut into 4 µm sections. Treatment of organotypic cultures was performed daily with 1 µM ATRA (Cat No. R2625; Sigma) in 100% ethanol or vehicle control. For organotypic cultures using PTX3 siRNA, stellate cells were first transfected with PTX3 siRNA or non-targeting (NT) control, harvested after 24 h and then plated into organotypic gels, and incubated for 5 days. Cancer cell lines (MiaPaCa2 or AsPC1) and stellate cell line (PS1: with NT siRNA or PTX3 siRNA) were used. Medium below the inserts were replaced daily and collected (undernatant) at the end of the cultures for further analysis. Each experiment had three technical replicates with three biological repeats.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!